FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l06n01_total_drought_1h_rep2_shoot.351000000672d8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l06n01_total_drought_1h_rep2_shoot.351000000672d8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59831212
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG4440620.7421912161832858No Hit
AACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTT2370790.3962463605116339No Hit
GCAACTGGATAACTAGCACCGAAAACCGTCTTTACATCGGATGGTTCGGT1807760.3021433027296856No Hit
AGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTCT1779680.29745010012499834No Hit
GCCCCTCCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTT1570530.2624934290149429No Hit
CCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTA990000.1654654764473098No Hit
TACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTC915110.15294859813302794No Hit
GTAGTTGATAGCCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAA883300.14763197509687753No Hit
GCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAA820660.13716252313257501No Hit
CTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCC813460.13595913784932187No Hit
AAACGGATTCAATTTCAACCAATCTGTAGTTGATAGCCAAGGTCGCGTTA755950.12634709789933724No Hit
ACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCG752440.1257604475737513No Hit
AAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTC677110.1131700290477151No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTGC1103100.056.4069187
TGTGCTC1337100.049.9569059
GGGTGTG631400.043.732556
AGCCTTC627550.038.717425
CGGTGTG317300.036.917486
CCTTCTA672350.036.010437
TAATACG1281750.035.8558468
CTAGTTA1317600.035.0154463
AATACGT1314950.034.8494079
AAGCCTT718300.033.9429664
TAGTTAA1414150.032.6176644
TTCTAGT1412450.032.2448581
GTTAATA1433550.032.1946536
GCCTTCT767550.031.7853836
GTGTGCT2763500.029.9296448
CTTCTAT812250.029.8375458
AGTTAAT1586400.029.1974935
AGGGTGT333950.028.3488185
TGGTGTG678800.027.9005996
TGGGTGT399850.027.4187585
Produced by FastQC (version 0.11.2)