FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l06n01_total_drought_1h_rep1_shoot.3510000006726e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l06n01_total_drought_1h_rep1_shoot.3510000006726e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57731125
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTT20347463.5245216510158084No Hit
TTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG9715231.6828409285285884No Hit
CTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCC2325980.4028987829355482No Hit
AGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT2228710.3860499860343965No Hit
TACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTC2209900.38279177826519056No Hit
ACTTAACCGGGGGATTCACCGCAAATACTAGTTTGGCTCATTATTGCCGC2086520.36142029104750684No Hit
GCAACTGGATAACTAGCACCGAAAACCGTCTTTACATCGGATGGTTCGGT1919120.33242380085265966No Hit
GCCCCTCCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTT1785030.30919716184294No Hit
AGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTCT1588910.27522588551669486No Hit
GCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAA1528690.2647947705851913No Hit
ATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATA1518950.2631076390768411No Hit
CCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTA1509850.2615313663123662No Hit
ACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTG1466830.2540795801225076No Hit
GACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCT1424000.24666070512223692No Hit
TTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAA1419560.24589162258660296No Hit
ACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCC1408890.24404339946605927No Hit
CAACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCT1291570.2237216059794435No Hit
TAACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCT1198100.2075310328700506No Hit
TGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTACGGAAACAATA1152980.19971549142685166No Hit
AAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTC1001420.17346275514291468No Hit
AAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCA955380.16548785425539517No Hit
GTATTGCAATTTGGTGGAGGAACTTTAGGACATCCTTGGGGTAATGCACC947090.1640518870886372No Hit
TAACCGGGGGATTCACCGCAAATACTAGTTTGGCTCATTATTGCCGCGAC908740.1574090232954927No Hit
TATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA898120.1555694610143142No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG881490.152688865841433TruSeq Adapter, Index 1 (100% over 49bp)
CCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTG860820.14910847484783987No Hit
AGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAA850130.14725678739155004No Hit
CACAAGGATGAAATATCAGTAATTTTCTATTTACTGGTTGATCCCATCTT819870.1420152474077025No Hit
GACTACTTAACCGGGGGATTCACCGCAAATACTAGTTTGGCTCATTATTG797030.13805897598565764No Hit
GGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTACGGAAACAATATTAT795280.137755846607874No Hit
TGCTCGTGAAGGTAATGAAATTATCCGATCAGCTTGCAAATGGAGTCCTG777380.13465526611511555No Hit
ATCGAGTAGACCCTGTCGTTGTGAGAATTCTTAATTCATGAGTTGTAGGG760040.13165168702324787No Hit
CACATGTTAGGTGTAGCTGGTGTATTCGGCGGTTCCCTATTCAGTGCTAT754890.13075962056862742No Hit
GGTTCTTTACTTTACGGAAACAATATTATCTCTGGTGCCATTATTCCTAC747730.12951938837152405No Hit
ACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCG744690.12899280933811702No Hit
CAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGT741670.12846969464045607No Hit
AAACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCT693860.12018820003940683No Hit
ATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAAC684840.11862578461791623No Hit
TGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCCTCCAGTA675550.11701660066385333No Hit
GTATTTATTATCGCCTTCATCGCTGCCCCTCCAGTAGATATTGATGGTAT646400.11196733131391429No Hit
CCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCCTCCAGTAGAT602410.1043475248403699No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCCTT3186850.064.216574
AACAAGC3168200.064.129881
AGCCTTC3215400.063.5814065
CCTTCTA3315850.061.6421177
GCCTTCT3459700.059.0999646
CTTCTAT3464350.059.0563358
GGTGTGC947100.052.4359667
TGTGCTC888600.049.9493949
TTCTATT4234650.048.3665129
ACAAGCC4360650.047.2348252
CAAGCCT4424800.046.6632163
GGGTGTG482300.044.8221326
CGGTGTG257250.035.6003766
CGAGTAG219850.035.065553
GTAGACC222450.035.0198556
TCTATTA3653700.032.937110-11
TAGACCC237650.032.679927
CTATTAT3441600.032.03682710-11
TTATCTT3761500.031.9680814-15
ATCTTTC3910650.030.76467916-17
Produced by FastQC (version 0.11.2)