FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l05n02_total_heat_1h_rep3_shoot.35200000067377.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l05n02_total_heat_1h_rep3_shoot.35200000067377.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39456196
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA4301391.0901684490821164No Hit
CTCTAGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA2648730.6713090131648778No Hit
CTCTATAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA2190430.5551548861932863No Hit
GCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG2066600.5237707152509076No Hit
CTCTGAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1974670.5004714595395867No Hit
CTCTACAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1858800.4711047157206944No Hit
CTCTCAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1823550.4621707576675663No Hit
CTCTTAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1769010.44834783363302433No Hit
GCCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1698870.4305711579494384No Hit
TGACCAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT1585550.4018507004577937No Hit
ACCGGCTCGAACTCGAATTTGATCGCTTTCCATATTTCACAAGCTGCGGC1305000.3307465321796354No Hit
GCCGCTGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1150750.291652545521621No Hit
TGGTTTATTCAAATTGCAAGGACTCCCAAGCACACGTATTAACTAGAAAG1090670.2764255327604313No Hit
TGACCGAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT959820.24326217357598284No Hit
CTCTGGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA926860.23490860598928492No Hit
GCCGCCGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG899370.22794138593593768No Hit
CCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT824500.20896591247671215No Hit
CCCCCAGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG821270.20814728312886524No Hit
GCCGTAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG735880.18650556176272035No Hit
CGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA692370.17547814290054725No Hit
CCCCCGGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG645500.16359914676011847No Hit
TGGCCAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT612330.1551923555935296No Hit
CTCAAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA564930.14317903327528078No Hit
CTCCAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA564210.14299655242994028No Hit
GCCGTGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG551660.13981580991740816No Hit
CTCGAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA546690.13855618519332175No Hit
CCCCCCGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG492280.12476620908918841No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC468390.11871139326254361Illumina Single End PCR Primer 1 (100% over 50bp)
GTCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG429540.10886503098271308No Hit
CCCCCTGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG428540.10861158536418464No Hit
TGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA405670.10281528406843883No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCAGT3281800.076.536699
ATTGCAG3311400.075.853388
CCGCGGA280300.070.4858552
GAATTGC721550.068.4296956
CATACGC597300.068.187479
CCCGGAC126950.066.727513
CGCGGAA339850.066.5481643
GCCGCGG268650.066.542981
CGGAAGT413550.063.7635845
AATCTGT723000.061.9706767
CTAGAAT465400.061.828553
TAATTGC465950.061.337676
CTCTAGA453200.060.493811
ACATACG676000.060.2955138
CTCTACA329850.059.350021
CGGACAT167900.058.725675
TCTGTAA773300.057.8656739
ATCTGTA773900.057.8550038
GCGGAAG450050.057.6471254
CCGGACA159400.057.590494
Produced by FastQC (version 0.11.2)