FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l05n02_total_heat_1h_rep2_shoot.3520000006730d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l05n02_total_heat_1h_rep2_shoot.3520000006730d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107944766
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG10261750.9506482231848091No Hit
GCCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG9024620.8360405357680798No Hit
TGACCAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT7826240.725022647230529No Hit
GCCGCTGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG6742170.6245944337866275No Hit
CTCTAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA6599640.6113904587092254No Hit
GCCGCCGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG5674400.525676251871258No Hit
ACCGGCTCGAACTCGAATTTGATCGCTTTCCATATTTCACAAGCTGCGGC5654370.5238206732506141No Hit
TGACCGAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT4967790.460215921909544No Hit
GCCGTAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG4462590.41341420852216215No Hit
TCAAAAATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATGGA4049630.3751576060667916No Hit
CCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT4006380.3711509273177729No Hit
CTCTAGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA3691010.3419350596396679No Hit
GCCGTGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG3582820.33191234116900115No Hit
GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT3575450.33122958458217416No Hit
CGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA3539510.32790010402171793No Hit
CTCTATAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA3440030.31868427969911944No Hit
CCCCCAGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG3264230.3023981727840329No Hit
TGGCCAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT3220950.29838871483588186No Hit
CTCTCAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA3165990.2932972220255682No Hit
CTCTACAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA3064440.2838896329628432No Hit
CTCTGAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA2957330.2739669656609381No Hit
CTCTTAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA2882940.2670754782126259No Hit
CCCCCGGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG2644750.24500956350213404No Hit
TGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA2303060.21335541178531992No Hit
GTCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG2280050.21122376605087087No Hit
CCCCCCGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG2215780.20526979510984347No Hit
GCTGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG2153160.1994686801210908No Hit
TCAAAGATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATGGA2133610.19765756868656328No Hit
GGAGGGGCAGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA1976750.18312606282364816No Hit
GGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA1968610.18237197345909298No Hit
CCCCCTGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG1847690.17116994815663408No Hit
GCCGGCTCGAACTCGAATTTGATCGCTTTCCATATTTCACAAGCTGCGGC1768100.1637967328587289No Hit
GCTGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1744310.16159282794684088No Hit
CTTCAAAAATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATG1710590.1584690081221724No Hit
CCGCAAAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT1696280.1571433301360809No Hit
CCGCATAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT1693770.1569108038086812No Hit
CCGCTGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT1601270.14834160648419026No Hit
AGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA1540890.14274800503064686No Hit
CCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT1536670.14235706435270792No Hit
CCGGGGTGTAGTAAGTCAATTTATAATCCTTAACACCAGCTTTAAATCCA1502020.13914708935493916No Hit
GTCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1460310.13528307616137683No Hit
CCGCCGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT1449430.13427515327607453No Hit
TCAAGAATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATGGA1428910.1323741810696037No Hit
TGACCTAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT1391410.12890018215426954No Hit
ACCGGTTCGAACTCGAATTTGATCGCTTTCCATATTTCACAAGCTGCGGC1347810.12486107941537435No Hit
CTCTGGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1324010.1226562481037756No Hit
ATGGTCATTGAGGGCCTCCTAAAAGGATCTACTAAATTCATCGAGTTGTT1286070.11914148760116819No Hit
GCCGTTGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1139550.10556787903917453No Hit
TGGCCGAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT1116180.10340288291513827No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCGA702150.075.272041
CCCGGAC498650.074.5234453
CCGCGGA1429250.074.16492
CGGAAGT2058550.072.142855
CGGACAT619400.072.1024255
ACCGAAT800600.071.149173
CGCGGAA1756950.070.709393
CGAATCT975600.070.55235
AATCTGT3508750.070.469097
GACCGAA774300.069.853352
GGACATA770200.069.750286
CATACGC2761950.069.307719
TTGCAGT6002800.069.11259
CCGAATC1009800.068.725514
ATTGCAG6077500.068.250088
GCCGCGG1399250.067.487791
CCGGACA605000.067.288494
GCGGAAG2163900.067.0110244
ATCTGTA3827700.064.6592568
TCTGTAA3846300.064.304749
Produced by FastQC (version 0.11.2)