FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l05n02_total_heat_1h_rep1_shoot.35200000067292.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l05n02_total_heat_1h_rep1_shoot.35200000067292.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31229783
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA2626740.8411009452098979No Hit
GCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG2328960.7457496582669179No Hit
TGACCAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT2152240.6891626496412095No Hit
GCCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1965700.6294312067426149No Hit
ACCGGCTCGAACTCGAATTTGATCGCTTTCCATATTTCACAAGCTGCGGC1458910.46715342210350935No Hit
CTCTAGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1454730.46581495619101804No Hit
GCCGCTGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1301430.4167271991611341No Hit
CTCTATAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1275910.4085555125375031No Hit
TGACCGAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT1213760.3886546377859878No Hit
CCCCCAGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG1169670.3745367042736096No Hit
CTCTGAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1115800.357287144774589No Hit
CTCTCAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1060420.33955407246985997No Hit
CTCTACAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1055990.33813555476834406No Hit
CTCTTAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1000110.3202423788855657No Hit
GCCGCCGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG957560.30661756439357907No Hit
CCCCCGGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG916180.2933673922742274No Hit
TGGCCAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT832980.266726156886841No Hit
CCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT832830.2666781258134262No Hit
GCCGTAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG810120.2594062212984317No Hit
CGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA724560.23200929702265305No Hit
CCCCCCGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG628950.2013942908280855No Hit
GCCGTGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG604150.1934531533568453No Hit
CCCCCTGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG573570.18366121852335637No Hit
CTCTGGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA460000.14729529180526166No Hit
GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT446040.14282519990612808No Hit
GTCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG441510.14137466148900235No Hit
ATGGTCATTGAGGGCCTCCTAAAAGGATCTACTAAATTCATCGAGTTGTT412960.13223274718239317No Hit
TGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA407330.1304299808935592No Hit
CCGGGGTGTAGTAAGTCAATTTATAATCCTTAACACCAGCTTTAAATCCA398490.12759934963364938No Hit
GCTGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG375740.1203146368324109No Hit
TCAAAAATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATGGA353920.11332771668634394No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC345640.11067640143384923Illumina Single End PCR Primer 1 (100% over 50bp)
GCCGGCTCGAACTCGAATTTGATCGCTTTCCATATTTCACAAGCTGCGGC342980.10982465039862747No Hit
GGAGGGGCAGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA335380.1073910760122797No Hit
GGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA332890.10659376019359469No Hit
CTCCAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA318560.10200519164670468No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGC685250.074.637729
CCCGGAC150000.074.517573
TTGCAGT1877000.073.970439
CCGCGGA298750.073.572312
ATTGCAG1898600.073.119148
CGCGGAA355150.070.299513
AATCTGT804400.069.4000857
GCCGCGG296800.069.38311
CGGAAGT416500.068.604495
CGGACAT185400.067.760355
ACATACG762450.067.059728
CCGGACA177950.066.987384
TGACCGA193450.065.657331
ATCTGTA859800.064.964468
TCTGTAA861350.064.844019
CCCCGGA171950.064.203092
GACCGAA203150.064.159462
GACATAC798850.063.98347
ACCGAAT210400.063.5521743
GCGGAAG451750.062.6458784
Produced by FastQC (version 0.11.2)