FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l05n02_total_ctrl_1h_rep3_shoot.35200000067235.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l05n02_total_ctrl_1h_rep3_shoot.35200000067235.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57022207
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA2619930.45945783894334363No Hit
TGACCAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT1855030.32531711724170903No Hit
CTCTAGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1790680.31403204018392344No Hit
TGGTTTATTCAAATTGCAAGGACTCCCAAGCACACGTATTAACTAGAAAG1386110.2430824888977026No Hit
CTCTGAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1358310.23820719531252096No Hit
CTCTATAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1316600.23089250123202004No Hit
CTCTCAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1266840.22216607645509057No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1133970.19886462830174217Illumina Single End PCR Primer 1 (100% over 50bp)
CTCTTAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1074980.18851953590642326No Hit
ACCGGCTCGAACTCGAATTTGATCGCTTTCCATATTTCACAAGCTGCGGC1005010.17624887791523047No Hit
GCTTGCAAATGGAGTCCTGAACTAGCCGCAGCTTGTGAAATATGGAAAGC986310.172969453812968No Hit
CTCTACAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA983930.17255207256358915No Hit
TCAAAAATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATGGA954990.16747685686736047No Hit
TGGCCAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT952870.16710507188892215No Hit
TGACCGAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT938680.16461656771720531No Hit
GCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG793840.13921593739786325No Hit
GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT783160.13734298288384383No Hit
GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG745090.13066663659651054No Hit
GGAGGGGCAGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA685520.12021982944293967No Hit
GCCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG684040.11996028143912423No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGC552600.062.3806579
TTGCAGT3068650.058.952439
ATTGCAG3159900.057.2581988
TCTGTAA1078550.054.773329
AATCTGT1100550.053.5953377
GAATTGC686700.052.963966
CCCGGAC116900.051.911933
ATCTGTA1137950.051.8786628
CCGCGGA181650.051.730422
CCGGCTC335450.051.4248772
CGGCTCG349250.050.1968153
ACATACG689300.050.1728488
CGAACTC464550.048.2266358
GCCGCGG158900.047.960291
TGACCGA212150.047.8738561
GCTCGAA402250.046.6519785
CGCGGAA244200.046.220473
GACATAC745100.046.1959467
CTCGAAC417450.046.139756
TCGAACT494150.045.9407737
Produced by FastQC (version 0.11.2)