FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l05n01_total_heat_1h_rep3_shoot.3510000006737a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l05n01_total_heat_1h_rep3_shoot.3510000006737a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39456196
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTT11513672.9180892146825306No Hit
TTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG7201981.8253102757295712No Hit
AGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT1663860.4216980268447572No Hit
ATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATA1067550.27056587006005345No Hit
CCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTA1045920.26508384133128293No Hit
GCCCCTCCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTT990560.25105309188954755No Hit
ACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTG885250.22436273380231586No Hit
CAACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCT790920.20045520860652657No Hit
TGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTACGGAAACAATA780970.19793342470216846No Hit
GGTTCTTTACTTTACGGAAACAATATTATCTCTGGTGCCATTATTCCTAC731340.18535491865460116No Hit
ACTTAACCGGGGGATTCACCGCAAATACTAGTTTGGCTCATTATTGCCGC712400.1805546586396722No Hit
GGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTACGGAAACAATATTAT690470.17499659622534317No Hit
TATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA676500.17145596093450063No Hit
TAACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCT636840.16140430770366204No Hit
AAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCA631350.16001289125794083No Hit
AGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTCT594070.1505644385992No Hit
AGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAA557900.1413973105770257No Hit
TACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTC553380.1402517363812771No Hit
ATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAAC531100.1346049680004631No Hit
GCAACTGGATAACTAGCACCGAAAACCGTCTTTACATCGGATGGTTCGGT529530.1342070583793734No Hit
TTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAA511540.1296475717020465No Hit
CTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCC504240.12779741868678876No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG482780.12235847571316809TruSeq Adapter, Index 12 (100% over 49bp)
AAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTC437190.11080388996445577No Hit
GCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAA436480.11062394357530057No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCCTT1857650.062.9125864
AACAAGC1853700.062.3759231
AGCCTTC1881800.062.1446465
CCTTCTA1932200.060.489237
GGTGTGC902050.059.255257
GCCTTCT2023150.057.8474356
CTTCTAT2021500.057.820568
TGTGCTC843250.054.398049
CAAGCCT2237800.052.488543
ACAAGCC2243400.052.2658162
GGGTGTG459500.049.7736936
TTCTATT2398500.048.845129
CGGTGTG220200.040.1869136
TGGTGTG560500.035.6606456
AGGGTGT225900.034.7572755
GTGGTGT470850.034.5616045
TGGGTGT261900.032.7908025
TCTATTA2091950.032.7485310-11
TGTGGTG460950.031.6964474
CTATTAT2024500.031.37364210-11
Produced by FastQC (version 0.11.2)