FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l05n01_total_heat_1h_rep2_shoot.35100000067300.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l05n01_total_heat_1h_rep2_shoot.35100000067300.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107944766
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG18211541.6871165388417257No Hit
AACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTT12841621.1896473053635597No Hit
GCAACTGGATAACTAGCACCGAAAACCGTCTTTACATCGGATGGTTCGGT8650530.8013848489884169No Hit
GCCCCTCCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTT7004830.6489272485893387No Hit
AGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTCT6094540.5645980093189512No Hit
CTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCC5954710.551644162163453No Hit
TACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTC5184110.48025580045261296No Hit
ACTTAACCGGGGGATTCACCGCAAATACTAGTTTGGCTCATTATTGCCGC4315080.3997488863888037No Hit
AAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTC4107140.38048533080334807No Hit
TTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAA3820560.35393656789250905No Hit
GCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAA3726930.34526268740070265No Hit
CCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTA3397480.31474244892985365No Hit
AGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT3386640.31373823164339437No Hit
GTAGTTGATAGCCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAA2962930.2744857494989613No Hit
ACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCG2759800.2556677921743793No Hit
AAACGGATTCAATTTCAACCAATCTGTAGTTGATAGCCAAGGTCGCGTTA2650610.24555243373263694No Hit
CAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGT2489990.23067260157847766No Hit
GACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCT2318630.21479781613496668No Hit
CACATGTTAGGTGTAGCTGGTGTATTCGGCGGTTCCCTATTCAGTGCTAT2222370.20588029251923154No Hit
ACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCC2116900.19610955476989037No Hit
TGCTCGTGAAGGTAATGAAATTATCCGATCAGCTTGCAAATGGAGTCCTG2053800.19026397259502142No Hit
CACAAGGATGAAATATCAGTAATTTTCTATTTACTGGTTGATCCCATCTT2018670.18700953041113638No Hit
CTGCCCCTCCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCT1996960.18499831663908559No Hit
TAACCGGGGGATTCACCGCAAATACTAGTTTGGCTCATTATTGCCGCGAC1964640.1820041927739229No Hit
TGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTACGGAAACAATA1898980.1759214522731005No Hit
CTGCAACTGGATAACTAGCACCGAAAACCGTCTTTACATCGGATGGTTCG1712560.1586515088651913No Hit
CCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTG1677520.15540540427870306No Hit
GGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTACGGAAACAATATTAT1656370.1534460688904546No Hit
ACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTG1650700.15292080025445606No Hit
AGCCGAGCCATTCATTCCTTTCTATGAAAGAAAGATAAGGGGACAGAACA1602820.1484851984393574No Hit
ATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATA1598460.1480812881654679No Hit
GACTACTTAACCGGGGGATTCACCGCAAATACTAGTTTGGCTCATTATTG1564520.14493708754716278No Hit
AGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAA1553480.1439143422664884No Hit
TACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAAC1526090.14137693345872832No Hit
GGGTATGCGTCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTG1519420.14075902485165423No Hit
TGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCCTCCAGTA1506400.1395528524282502No Hit
TCACATGTTAGGTGTAGCTGGTGTATTCGGCGGTTCCCTATTCAGTGCTA1458460.135111692214887No Hit
GTATTGCAATTTGGTGGAGGAACTTTAGGACATCCTTGGGGTAATGCACC1448820.13421864289371843No Hit
GCTGCCCCTCCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTC1431360.13260114899873884No Hit
CAACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCT1408680.13050007445474476No Hit
CCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCCTCCAGTAGAT1373120.1272057970832972No Hit
TAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTC1330010.12321208793022907No Hit
GCTAATGAGGGTTACAGATTTGGTCAAGAGGAAGAAACTTATAATATTGT1296710.12012717689341232No Hit
GTAGCTGGTGTATTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTT1270500.11769908325152142No Hit
ATCGAGTAGACCCTGTCGTTGTGAGAATTCTTAATTCATGAGTTGTAGGG1248780.11568694307975988No Hit
GTATTTATTATCGCCTTCATCGCTGCCCCTCCAGTAGATATTGATGGTAT1156850.10717055053878202No Hit
GCTTGGCCTGTAGTAGGGATTTGGTTCACTGCTTTAGGTATTAGTACTAT1154990.10699824019258146No Hit
CGGGTAGGTTGTGGTATTTCATTGCTACAAACATGGGTTATTCTAAAATA1148770.10642201957249135No Hit
TATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCCTCCA1123740.10410324109646964No Hit
ATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAAC1116140.10339917731629526No Hit
AAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCA1094690.10141204993672412No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCTTC2569250.051.189965
AAGCCTT2599050.050.5894434
CCTTCTA2691900.048.8011447
AACAAGC2795550.046.7860451
CTTCTAT2900800.045.3239758
TAGCCTG327300.043.1513821
GCCTTCT3067550.042.9689986
GGTGTGC1151900.040.678627
CCGAGCC392150.039.9948163
TGTGCTC911700.038.585789
CGAGCCA421600.037.3586354
CGAGTAG365250.035.5148773
CTAGTTA5396600.034.3779343
CGGGTAG384400.033.9485441
TAGTTAA5501850.033.7365464
GTAGACC384800.033.698576
ACTTAAC1264050.033.6322631
AGCCGAG492750.033.4829751
TTCTAGT5621700.032.589881
TAATACG5761750.032.2861988
Produced by FastQC (version 0.11.2)