FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l05n01_total_heat_1h_rep1_shoot.35100000067295.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l05n01_total_heat_1h_rep1_shoot.35100000067295.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31229783
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTT5272821.6883946968187389No Hit
TTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG4148651.3284274181476061No Hit
ACTTAACCGGGGGATTCACCGCAAATACTAGTTTGGCTCATTATTGCCGC1213310.3885105445657435No Hit
CTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCC1018600.32616300920182506No Hit
GCCCCTCCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTT1003450.3213118707869344No Hit
GCAACTGGATAACTAGCACCGAAAACCGTCTTTACATCGGATGGTTCGGT958310.30685771976065285No Hit
AGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT828860.2654069034037156No Hit
TACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTC688050.22031853375350063No Hit
TTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAA680830.21800663808647022No Hit
ATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATA671840.21512797575314563No Hit
CCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTA656700.21028003940981596No Hit
AGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTCT638590.204481087812874No Hit
ACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCC547930.17545110704099354No Hit
TATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA533310.170769678418835No Hit
GCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAA527260.1688324251244397No Hit
AAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTC523820.16773091250746122No Hit
CAACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCT506560.16220413699320294No Hit
TAACCGGGGGATTCACCGCAAATACTAGTTTGGCTCATTATTGCCGCGAC491540.15739462550860506No Hit
TGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTACGGAAACAATA471440.1509584616710273No Hit
ACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTG449300.14386907523500883No Hit
GGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTACGGAAACAATATTAT443950.14215596694988242No Hit
CACATGTTAGGTGTAGCTGGTGTATTCGGCGGTTCCCTATTCAGTGCTAT423180.13550526431771875No Hit
AGCCGAGCCATTCATTCCTTTCTATGAAAGAAAGATAAGGGGACAGAACA413350.13235762797327155No Hit
ATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAAC408300.13074058183497464No Hit
CAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGT390370.12499926752613043No Hit
TAACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCT389460.12470787901408088No Hit
ACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCG382420.1224536206351482No Hit
GACTACTTAACCGGGGGATTCACCGCAAATACTAGTTTGGCTCATTATTG362090.11594380915166781No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG354610.11354865962405183TruSeq Adapter, Index 5 (100% over 49bp)
CACAAGGATGAAATATCAGTAATTTTCTATTTACTGGTTGATCCCATCTT341200.10925468166077235No Hit
GACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCT323980.10374071443275798No Hit
ATCGAGTAGACCCTGTCGTTGTGAGAATTCTTAATTCATGAGTTGTAGGG317710.10173301556402105No Hit
TGCTCGTGAAGGTAATGAAATTATCCGATCAGCTTGCAAATGGAGTCCTG314780.10079480859665275No Hit
TCACATGTTAGGTGTAGCTGGTGTATTCGGCGGTTCCCTATTCAGTGCTA314690.10076598995260388No Hit
AAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCA312950.10020882950099268No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCTTC984450.054.5848245
AAGCCTT991950.054.3189934
CCTTCTA1009000.053.1625677
AACAAGC1055450.050.6410181
CTTCTAT1093250.049.123638
GCCTTCT1127350.047.7037126
GGTGTGC354750.045.6181347
TGTGCTC307050.040.8564879
ACAAGCC1458450.037.4054532
CAAGCCT1479500.037.025823
TTCTATT1482250.036.3374949
GTAGACC109650.032.8358576
GGGTGTG207450.032.4218376
CGAGTAG112450.032.0174753
TTAACCG370050.031.7039873
CCGAGCC133950.031.6654823
ACTTAAC378250.030.9464441
CGAGCCA137600.030.8253694
TAGACCC119300.030.1798117
CTTAACC402000.029.315422
Produced by FastQC (version 0.11.2)