FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l05n01_total_ctrl_1h_rep3_shoot.35100000067238.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l05n01_total_ctrl_1h_rep3_shoot.35100000067238.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57022207
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG11082691.9435743691926903No Hit
AACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTT6711661.1770256454647572No Hit
AGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT2070870.3631690369332776No Hit
GCCCCTCCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTT1903230.33376996439299517No Hit
TGCTCGTGAAGGTAATGAAATTATCCGATCAGCTTGCAAATGGAGTCCTG1549190.271681872993797No Hit
GCAACTGGATAACTAGCACCGAAAACCGTCTTTACATCGGATGGTTCGGT1508200.26449344550974674No Hit
AGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTCT1432380.25119687142239167No Hit
CACAAGGATGAAATATCAGTAATTTTCTATTTACTGGTTGATCCCATCTT1175460.20614074092221651No Hit
ACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCG1150530.20176876002010935No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG1133450.19877343575986106TruSeq Adapter, Index 4 (100% over 49bp)
CCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTA1124080.19713021630327288No Hit
GTAGTTGATAGCCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAA1006410.17649439629721803No Hit
ATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATA849570.1489893227036968No Hit
ACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTG822020.14415787168672722No Hit
TACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTC767560.13460720662741096No Hit
CAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGT713100.12505654156809468No Hit
ATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAAC662140.11611967246374733No Hit
TATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA581070.10190240444393883No Hit
ACCGGCTCGAACTCGAATTTGATCGCTTTCCATATTTCACAAGCTGCGGC573040.1004941811529673No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCTTC1161650.057.8543055
AACAAGC1201550.055.8020631
CCTTCTA1231450.054.5904857
AAGCCTT1238350.054.4196554
GCCTTCT1338850.050.3461576
CTTCTAT1358600.049.640758
CTAGTTA2750650.041.6904873
GCTCGTG383450.041.4239122
TAGTTAA2816850.040.6969264
CTCGTGA392400.040.4658283
TTCTAGT2814900.040.2906461
TAATACG2894800.039.613168
AATACGT2921100.039.2142229
TGCTCGT411450.038.7708741
GTTAATA3032600.037.908746
TCGTGAA425300.037.4245874
CGTGAAG435250.037.235355
ACAAGCC1844150.037.0516242
AGTTAAT3155150.036.4634135
CAAGCCT1903250.036.114683
Produced by FastQC (version 0.11.2)