FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l04n02_total_salt24h_rep3_shoot.352000000672af.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l04n02_total_salt24h_rep3_shoot.352000000672af.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41344847
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA3172600.767350765622618No Hit
TGGTTTATTCAAATTGCAAGGACTCCCAAGCACACGTATTAACTAGAAAG2803240.6780143605320392No Hit
CTCTAGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA2226580.538538696249136No Hit
CTCTATAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1736270.41994834326028585No Hit
CTCTGAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1721850.41646060511482846No Hit
CTCTCAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1653890.40002324836272823No Hit
CTCTACAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1521880.3680942391684265No Hit
CTCTTAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1488660.3600593805559372No Hit
TGACCAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT1450690.3508756484211926No Hit
GCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1128360.2729143005414919No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1050560.25409696158749845Illumina Single End PCR Primer 1 (100% over 50bp)
TGACCGAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT949450.22964167699060536No Hit
GCCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG915150.22134560081937177No Hit
GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG857910.2075010702059195No Hit
CTCTGGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA845500.20449948696145856No Hit
TCAAAAATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATGGA831010.2009948180483048No Hit
GGAGGGGCAGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA770140.18627230619573948No Hit
GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT722330.17470859185910156No Hit
GCCGCTGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG688070.16642219041226589No Hit
CTCCAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA683710.1653676454528904No Hit
TGGCCAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT679760.16441226641859383No Hit
CCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT679470.16434212466670878No Hit
TGGTTCATTCAAATTGCAAGGACTCCCAAGCACACGTATTAACTAGAAAG629780.1523236982833677No Hit
CGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA627980.1518883356854604No Hit
GCCGCCGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG591800.14313754746752358No Hit
GCCGTAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG557030.13472779328461418No Hit
GCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGACTCCCAAGCA530830.12839084880396343No Hit
CTCAAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA499950.12092196156875366No Hit
CTCGAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA490150.11855165409125834No Hit
CACAGGCTTGTACTTTCGCGTCTCTCTAAAATTGCAGTCATGGTAAGATC449210.10864957366996666No Hit
CCACAGGCTTGTACTTTCGCGTCTCTCTAAAATTGCAGTCATGGTAAGAT430980.10424031802560546No Hit
TGGTCTATTCAAATTGCAAGGACTCCCAAGCACACGTATTAACTAGAAAG426770.10322205328272228No Hit
GCCGTGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG414760.10031721728224074No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATTGC772300.066.409436
GCCGCGG159150.064.4109041
CCGCGGA198700.062.416022
TGACCGA170500.062.156021
CTAGAAT433700.060.3284033
TTGCAGT4143850.060.1766329
CTCTAGA400050.059.6871341
ATTGCAG4205450.059.2839938
CGCGGAA257250.059.0498163
TAATTGC489250.058.952846
CGGAAGT326950.058.332055
GACCGAA186000.057.7176132
AATCTGT924650.057.55467
CGAATCT251450.057.1683545
TAGAATT516750.056.3519824
CCGAATC257500.056.3221664
CTCTACA292650.055.762781
CTCTATA333800.055.7303161
CTATAAT359150.055.4778063
ACCGAAT199800.054.710353
Produced by FastQC (version 0.11.2)