FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l04n02_total_salt24h_rep2_shoot.35200000067242.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l04n02_total_salt24h_rep2_shoot.35200000067242.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50841444
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA6817181.3408706487565538No Hit
TGACCAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT4419840.8693380148683424No Hit
GCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG4364220.8583981210289778No Hit
CTCTAGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA4279420.8417188150674871No Hit
GCCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG3798150.7470578530381631No Hit
CTCTATAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA3618500.7117225073308303No Hit
CTCTGAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA3280880.6453160535723572No Hit
CTCTCAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA3210050.631384505916079No Hit
CTCTACAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA3154760.6205095197532156No Hit
CTCTTAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA2994900.5890666677366598No Hit
GCCGCTGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG2811720.5530370065806943No Hit
TGACCGAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT2605560.5124874108611077No Hit
GCCGCCGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG2372840.4667137306328278No Hit
GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT1857390.3653299068374219No Hit
GCCGTAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1855770.36501126915278015No Hit
TGGCCAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT1805430.35510989813743293No Hit
TGGTTTATTCAAATTGCAAGGACTCCCAAGCACACGTATTAACTAGAAAG1756110.3454091508494527No Hit
CTCTGGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1651810.32489439127653413No Hit
CCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT1624130.3194500140475947No Hit
CGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA1563670.30755814095288087No Hit
TCAAAAATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATGGA1482550.2916026539293416No Hit
GCCGTGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1452400.285672452576288No Hit
CTCCAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1156740.22751910822989213No Hit
TGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA985660.19386939521229962No Hit
CTCGAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA939040.18469971073205552No Hit
GTCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG935990.18409980644924248No Hit
CTCAAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA934160.18373986387955465No Hit
GCTGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG861760.1694995130350743No Hit
TCAAAGATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATGGA815260.16035343134628513No Hit
GGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA799430.15723982977352097No Hit
GGAGGGGCAGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA772460.15193510239402327No Hit
TGACCTAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT758410.14917160889450742No Hit
GCTGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG706430.13894766639594264No Hit
ACCGGCTCGAACTCGAATTTGATCGCTTTCCATATTTCACAAGCTGCGGC685630.1348565158770864No Hit
CCGCAAAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT677690.13329479784248455No Hit
CCGCATAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT668990.13158359546200143No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC657880.1293983703531316Illumina Single End PCR Primer 1 (100% over 50bp)
CTTCAAAAATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATG657610.12934526407235797No Hit
CCGCTGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT634370.12477419012725131No Hit
CCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT618340.12162125056872893No Hit
GTCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG611030.1201834471892655No Hit
AGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA591240.11629095349848836No Hit
CCGCCGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT588100.1156733471220841No Hit
TGGCCGAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT576390.11337010805593956No Hit
CTCTCGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA546430.10747727778935626No Hit
TCAAGAATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATGGA535360.10529992027763806No Hit
GGGTATGCGTCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTG511760.10065803795816658No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCGA356300.075.1450651
ATTGCAG5939250.074.409988
TTGCAGT5953000.074.250479
CCGCGGA596100.072.9785462
CGGAAGT848750.072.116825
CGAATCT489000.071.865435
GACCGAA380400.071.846282
CGCGGAA722500.070.475613
GAATTGC1234750.070.07756
CTCTAGA622700.070.065391
AATCTGT1878800.069.981757
ACCGAAT399450.069.792713
CTCTATA544500.069.664051
CCGAATC504700.069.53424
CTATAAT573900.069.172013
CTAGAAT689450.068.1993943
TAATTGC771700.067.9444056
CTCTACA479350.067.4739461
TAGAATT752100.067.043164
TCTATAA580850.066.720092
Produced by FastQC (version 0.11.2)