FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l04n02_total_ctrl_1h_rep1_shoot.35200000067384.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l04n02_total_ctrl_1h_rep1_shoot.35200000067384.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60650687
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG6029860.9941948390460935No Hit
GCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG5873530.96841936844013No Hit
GCCGCTGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG5271650.8691822402605267No Hit
GCCGCCGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG3994490.6586058950989294No Hit
GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT2995260.4938542575783189No Hit
GCCGGCTCGAACTCGAATTTGATCGCTTTCCATATTTCACAAGCTGCGGC2071710.34158063205450584No Hit
ACCGGCTCGAACTCGAATTTGATCGCTTTCCATATTTCACAAGCTGCGGC2043950.3370036022840104No Hit
GGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA1965390.32405073993638356No Hit
GGAGGGGCAGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA1938680.31964683268962807No Hit
CGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA1889600.311554591294242No Hit
CCCCCCGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG1818510.2998333720440792No Hit
CCCCCAGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG1355710.22352755872328373No Hit
GGGGGGGCAGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA1346280.2219727535815052No Hit
GCCGTGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1228910.2026209529992628No Hit
CCCCCTGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG1174400.19363342083825036No Hit
CCCCCGGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG1036370.17087522850318249No Hit
TGACCAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT859640.14173623457884327No Hit
GCCGGAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG814260.13425404398139795No Hit
CCGGGGTGTAGTAAGTCAATTTATAATCCTTAACACCAGCTTTAAATCCA796730.13136372222791146No Hit
CCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT739060.12185517370973886No Hit
TGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA728010.12003326524561873No Hit
GCCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA719270.11859222633372644No Hit
TGGCCAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT639230.10539534366692334No Hit
GCCGTAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG631110.10405652948333463No Hit
TGGCCGAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT619740.10218185986912234No Hit
GTCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG610570.10066992316179371No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGGA826050.078.1605152
CCGCTGA736350.075.804342
CGCTGAA785000.072.879113
CGGAAGT1094300.072.560515
GCCGCGG875950.071.971391
CGACATA371250.067.047836
CGCGGAA1062550.066.829133
GCCGCTG850800.066.0237961
CATACGC1259400.065.982119
CCGAAGT704950.065.527125
CCCCCGA320750.065.222372
CTGAAGT915200.064.281455
CCCCGAC317150.064.20193
CCCGGAC213250.062.4822163
CCCCCCG346450.062.410691
GCCGGCT422350.062.3917271
CCCGACA343700.061.927644
CGAACTC1089000.061.9055868
GCTGAAG960300.061.340464
GCGGAAG1295300.061.3399934
Produced by FastQC (version 0.11.2)