FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l04n02_total_ctrl_025h_rep3_shoot.3520000006731a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l04n02_total_ctrl_025h_rep3_shoot.3520000006731a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57242434
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA6652861.1622252121564223No Hit
TGACCAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT5896371.0300697555942504No Hit
GCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG5314960.9285000005415563No Hit
GCCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG4619810.8070603706334359No Hit
CTCTAGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA4054520.7083067082716993No Hit
TGACCGAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT3639510.6358062971256603No Hit
CTCTATAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA3532080.617038751357079No Hit
GCCGCTGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG3465670.6054372181308713No Hit
CTCTCAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA3241110.566207579502996No Hit
CTCTGAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA3186370.556644743652934No Hit
CTCTACAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA3090180.5398407761626628No Hit
CTCTTAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA2925410.5110561860454781No Hit
GCCGCCGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG2847040.4973652937259796No Hit
TGGCCAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT2449330.42788711605100505No Hit
ACCGGCTCGAACTCGAATTTGATCGCTTTCCATATTTCACAAGCTGCGGC2328720.4068170825859711No Hit
GCCGTAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG2327810.4066581096114815No Hit
GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT2253910.3937481065183217No Hit
CCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT2112010.3689588042325384No Hit
CGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA1955270.3415770195935414No Hit
GCCGTGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1824730.31877225905523165No Hit
TCAAAAATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATGGA1749450.30562117606669204No Hit
CTCTGGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1468020.25645660001110365No Hit
CCCCCAGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG1381880.24140832306327156No Hit
TGGTTTATTCAAATTGCAAGGACTCCCAAGCACACGTATTAACTAGAAAG1281120.2238059967890254No Hit
GTCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1248420.21809345144198447No Hit
TGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA1237010.2161001749156928No Hit
CTCCAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1166610.20380160633980032No Hit
CCCCCGGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG1131160.19760864815776352No Hit
GCTGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1107730.1935155308035993No Hit
GGAGGGGCAGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA1083140.18921976658085504No Hit
GGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA1018780.17797635928618968No Hit
CCCCCCGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG976740.17063215725592662No Hit
TGACCTAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT968150.16913152225497607No Hit
CCGGGGTGTAGTAAGTCAATTTATAATCCTTAACACCAGCTTTAAATCCA962790.1681951539656752No Hit
TCAAAGATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATGGA952340.16636958519269113No Hit
CTCAAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA917550.16029192609105336No Hit
GCTGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG899080.1570652987956452No Hit
CTCGAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA871200.1521947861266696No Hit
CCGCATAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT866310.15134052475825888No Hit
CCGCAAAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT863690.15088282234819017No Hit
CCGCTGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT821610.1435316324948726No Hit
CTTCAAAAATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATG818460.14298134142933194No Hit
CCCCCTGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG810410.141575042039617No Hit
CCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT803450.14035916082813668No Hit
TGGCCGAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT792260.1384043173286447No Hit
GCCGGCTCGAACTCGAATTTGATCGCTTTCCATATTTCACAAGCTGCGGC786460.13739108298574446No Hit
GTCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG779480.13617170786273694No Hit
ATGGTCATTGAGGGCCTCCTAAAAGGATCTACTAAATTCATCGAGTTGTT750310.1310758379002542No Hit
CCGCCGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT745580.13024952782406143No Hit
AGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA739390.12916816220637997No Hit
TGACTAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT630880.1102119452153275No Hit
TCAAGAATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATGGA627780.10967038892860496No Hit
GGGGGGGCAGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA580050.10133216906884149No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCGA468500.082.161351
ACCGAAT521950.077.38543
CGAATCT656600.075.761995
GACCGAA519050.075.478742
CCGCGGA732150.075.209522
CCGAATC662650.075.040384
AATCTGT2421450.074.0014347
TTGCAGT5849850.073.688339
CGGAAGT1056500.073.5745
CCCGGAC225000.073.44453
ATTGCAG5870550.073.402178
GAATCTG715800.072.302326
CTCTACA454350.072.1902851
CGGACAT278100.072.1308755
GCCGCGG682550.071.4836351
CGCGGAA910100.071.131983
TAATTGC722400.070.596426
CTCTATA529950.069.2656251
CTATAAT556000.069.067733
GGACATA356550.068.7331546
Produced by FastQC (version 0.11.2)