FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l04n01_total_salt24h_rep3_shoot.351000000672a2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l04n01_total_salt24h_rep3_shoot.351000000672a2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41344847
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG15403423.7255960821429577No Hit
AACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTT11038102.6697643844225616No Hit
AGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT2296900.5555468617407147No Hit
GCCCCTCCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTT1689370.4086047289037011No Hit
CCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTA1558750.37701191638222775No Hit
GGTTCTTTACTTTACGGAAACAATATTATCTCTGGTGCCATTATTCCTAC1434800.34703236415411093No Hit
ACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTG1402150.3391353703642923No Hit
ATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATA1397950.33811952430250863No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG1252610.3029664132025933TruSeq Adapter, Index 6 (100% over 49bp)
AGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTCT1102880.26675150110000406No Hit
AAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCA970090.23463383477994246No Hit
GCAACTGGATAACTAGCACCGAAAACCGTCTTTACATCGGATGGTTCGGT897120.21698471879700026No Hit
TGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTACGGAAACAATA850540.20571850223559904No Hit
AGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAA798470.19312442975058053No Hit
TACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTC742790.17965721338864793No Hit
CAACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCT719570.1740410358756437No Hit
CTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCC688410.16650442556964837No Hit
GTAGTTGATAGCCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAA649730.1571489670768403No Hit
AAACGGATTCAATTTCAACCAATCTGTAGTTGATAGCCAAGGTCGCGTTA633200.15315088721939157No Hit
GGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTACGGAAACAATATTAT629900.15235272245656153No Hit
GCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAA611760.14796523494209568No Hit
CTGGTTCTTTACTTTACGGAAACAATATTATCTCTGGTGCCATTATTCCT604540.1462189471882675No Hit
ACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCG564720.13658775905011816No Hit
AGACCTAGCTGCTCTTGAAGTTCCATCTCTTAATGGATAAGGCTTTTCTG532840.12887700370495989No Hit
GACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCT504100.12192571422503995No Hit
TCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGA490060.11852988596136296No Hit
ATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAAC475060.11490186431213545No Hit
CACATGTTAGGTGTAGCTGGTGTATTCGGCGGTTCCCTATTCAGTGCTAT452660.10948401864928899No Hit
CACAAGGATGAAATATCAGTAATTTTCTATTTACTGGTTGATCCCATCTT449440.10870520333525482No Hit
GTATTTATTATCGCCTTCATCGCTGCCCCTCCAGTAGATATTGATGGTAT445750.10781271000954483No Hit
TATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA436300.10552705637053149No Hit
ACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCC433170.10477000918639268No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAAGC1624850.067.908761
AAGCCTT1798050.061.965214
AGCCTTC1808550.061.666685
CCTTCTA1858950.060.2022487
GGTGTGC516250.059.4097027
GCCTTCT1919700.058.3564956
CTTCTAT1921300.058.2325948
TTCTATT2170000.051.6549769
TGTGCTC659950.051.534579
GGGTGTG325950.048.4974636
CAAGCCT2575500.043.635863
ACAAGCC2629500.042.6580962
CTAGTTA3953300.040.5800973
TTCTAGT3932400.040.5640831
TAGTTAA3997000.040.2279054
TAATACG4175700.038.6331948
AATACGT4200150.038.398139
GTTAATA4243000.038.0396966
AGTTAAT4336250.037.1523365
CGGTGTG159750.036.601926
Produced by FastQC (version 0.11.2)