FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l04n01_total_salt24h_rep2_shoot.35100000067245.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l04n01_total_salt24h_rep2_shoot.35100000067245.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50841444
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTT17481433.4384212218677344No Hit
TTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG12028982.365979219630347No Hit
AGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT2877700.5660146080823354No Hit
TATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA2817340.5541424039805006No Hit
ATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATA2628160.5169326032517881No Hit
AGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTCT2352170.4626481498047144No Hit
TACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTC2170640.42694302703125425No Hit
GCAACTGGATAACTAGCACCGAAAACCGTCTTTACATCGGATGGTTCGGT2136410.42021033076873265No Hit
ATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAAC1862410.36631729027995347No Hit
CTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCC1774370.34900070894917934No Hit
CAACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCT1730070.3402873451037307No Hit
GCCCCTCCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTT1626160.3198492946030408No Hit
TAACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCT1592910.31330935447073455No Hit
GCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAA1552820.30542405522549676No Hit
TTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAA1502340.29549514762011875No Hit
CCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTA1365970.2686725420308676No Hit
AAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTC1243350.24455442296249494No Hit
TGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTACGGAAACAATA1228590.24165127961353733No Hit
ACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTG1182240.23253470141406685No Hit
GACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCT1159100.2279832964618393No Hit
ACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCC1115170.2193427078900434No Hit
TCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGA1062500.20898304934061276No Hit
AAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCA993910.19549208712482674No Hit
ACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCG986260.19398740916957433No Hit
CACATGTTAGGTGTAGCTGGTGTATTCGGCGGTTCCCTATTCAGTGCTAT975990.19196740360088907No Hit
AAACGGATTCAATTTCAACCAATCTGTAGTTGATAGCCAAGGTCGCGTTA910930.17917075683373587No Hit
GGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTACGGAAACAATATTAT902580.17752839592832967No Hit
GTAGTTGATAGCCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAA866210.170374783218195No Hit
AGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAA862430.1696312952873644No Hit
CAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGT832980.16383877688446458No Hit
AAACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCT811950.15970238768198639No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG768200.1510972032973729TruSeq Adapter, Index 5 (100% over 49bp)
GGTTCTTTACTTTACGGAAACAATATTATCTCTGGTGCCATTATTCCTAC735140.14459463425153699No Hit
GTATTTATTATCGCCTTCATCGCTGCCCCTCCAGTAGATATTGATGGTAT709370.13952593478658867No Hit
TCACATGTTAGGTGTAGCTGGTGTATTCGGCGGTTCCCTATTCAGTGCTA688120.13534627379977643No Hit
TGCACTTTTACCCAATTTGGGAAGCTGCATCTGTTGATGAGTGGTTATAC611130.12020311618214463No Hit
TCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGA590840.11621227752697189No Hit
TAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTC588130.11567924781994783No Hit
TTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAA565020.11113374356558402No Hit
AGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAA557480.10965070150249863No Hit
CCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTG551150.10840565425325056No Hit
TACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAAC539430.10610044828781809No Hit
TAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTAC523950.10305568819013086No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAAGC2874200.060.6651231
AAGCCTT3018450.058.3913154
AGCCTTC3061550.057.6124845
CCTTCTA3135500.056.4652757
CTTCTAT3227200.054.880838
GCCTTCT3264200.054.2898036
GGTGTGC741250.050.8162237
TTCTATT3658400.048.477239
TGTGCTC752600.047.9868169
GGGTGTG383050.042.483966
ACAAGCC4265300.041.758762
CAAGCCT4344750.041.115293
TAGCCTG158400.039.7720871
TAACAAG434850.036.972681
TCTATTA3342900.033.46645410-11
CGGTGTG214850.031.9908836
CGACGCT190100.031.4089878
TAAGCCT132550.029.8576281
TTATCTT3754400.029.74184414-15
GACGCTC195650.029.5226219
Produced by FastQC (version 0.11.2)