FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l04n01_total_ctrl_1h_rep1_shoot.35100000067387.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l04n01_total_ctrl_1h_rep1_shoot.35100000067387.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60650687
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTCT5533510.9123573488953225No Hit
GCCCCTCCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTT4385490.7230734253677951No Hit
AAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTC3123790.5150461032700256No Hit
TACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTC3001780.4949292660114468No Hit
GCAACTGGATAACTAGCACCGAAAACCGTCTTTACATCGGATGGTTCGGT2760680.4551770369888803No Hit
ACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCG2589560.4269630119770943No Hit
CTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCC2356640.38855948985375877No Hit
ACGCGGTGGACTTGATTTTACCAAAGATGATGAAAACGTAAACTCACAAC2169980.357783251490622No Hit
ACTTAACCGGGGGATTCACCGCAAATACTAGTTTGGCTCATTATTGCCGC2139380.3527379665130586No Hit
CCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTA2002880.33023203842686893No Hit
CAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGT1746850.2880181719953147No Hit
GCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAA1472160.2427276709990111No Hit
GGGTATGCGTCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTG1425500.2350344358011971No Hit
ACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCCTCCAGTAGA1367040.22539563319373448No Hit
TGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCCTCCAGTA1130860.18645460685383497No Hit
GATTGCACTTTTACCCAATTTGGGAAGCTGCATCTGTTGATGAGTGGTTA1099250.18124279449629319No Hit
GCTACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGTTTCTCTGA1059130.17462786530348784No Hit
GCTTGGCCTGTAGTAGGGATTTGGTTCACTGCTTTAGGTATTAGTACTAT993910.16387448340032817No Hit
GACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCT987810.16286872397669624No Hit
TAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTC873270.14398352981558146No Hit
CCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTG869530.14336688387387928No Hit
CTGCCCCTCCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCT833710.13746093263543743No Hit
AGACCCTGTCGTTGTGAGAATTCTTAATTCATGAGTTGTAGGGAGGGACG826130.13621115289262922No Hit
CAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTC820620.13530267183947975No Hit
TTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAA814010.13421282433288842No Hit
TGGTGTAGCTTGTTATATGGGTCGTGAGTGGGAACTGAGTTTCCGTCTGG807250.13309824503719142No Hit
TACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAAC796480.13132250257940195No Hit
GGTACAGTAGTAGGTAAGTTAGAAGGGGAACGCGAAATGACTTTAGGTTT758840.12511647229981088No Hit
GTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTG739530.12193266664893672No Hit
ATCCACGCTGGTACAGTAGTAGGTAAGTTAGAAGGGGAACGCGAAATGAC738360.12173975869391224No Hit
AACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTT735070.12119730811952716No Hit
CCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCCTCCAGTAGAT717040.11822454706902166No Hit
AGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAA711440.11730122694240874No Hit
GCGGCGATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCTGTTGA707970.11672909822109682No Hit
TAACCGGGGGATTCACCGCAAATACTAGTTTGGCTCATTATTGCCGCGAC705020.11624270636868465No Hit
ACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCC655600.10809440625132573No Hit
GACTACTTAACCGGGGGATTCACCGCAAATACTAGTTTGGCTCATTATTG647400.10674240178021398No Hit
GCTAATGAGGGTTACAGATTTGGTCAAGAGGAAGAAACTTATAATATTGT639950.10551405625463073No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTG488350.045.8053362
ACGCGGT493700.044.5745161
TAGCCTG226350.040.467631
GGTGGAC602800.037.924925
GTGGACT601450.037.6154866
ACTTAAC573850.036.941321
TTAACCG582350.036.443113
GACTTGA645200.034.9846959
CGGTGGA675950.033.7008554
CTTAACC631750.033.5959172
GGACTTG696250.032.5627868
AACCGGG662250.032.5336765
GCGGTGG708200.032.2343
TAACCGG670400.031.8047474
ACCGGGG703000.030.8642966
CTGTCGT278350.030.4260646
CAGCCTG314350.030.2574161
CCATGAC1009100.030.1347663
CCTGTCG281150.030.0045365
TGTCGTT282300.029.949887
Produced by FastQC (version 0.11.2)