FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l04n01_total_ctrl_025h_rep3_shoot.3510000006731d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l04n01_total_ctrl_025h_rep3_shoot.3510000006731d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57242434
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG15067902.6322954750666265No Hit
AACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTT14799132.5853425450077823No Hit
AGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT3334760.5825678202293075No Hit
GCAACTGGATAACTAGCACCGAAAACCGTCTTTACATCGGATGGTTCGGT3038480.5308090148647417No Hit
AGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTCT2989030.5221703186136355No Hit
GCCCCTCCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTT2736780.4781033594762934No Hit
CTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCC2448120.42767573440360696No Hit
TACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTC2246180.39239770971304255No Hit
TATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA2136190.3731829432689742No Hit
TTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAA2109010.3684347175034521No Hit
ATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATA2097870.36648860878277817No Hit
AAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTC1904670.33273742342961865No Hit
ATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAAC1734230.3029623093944608No Hit
CCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTA1716030.29978284990467036No Hit
ACTTAACCGGGGGATTCACCGCAAATACTAGTTTGGCTCATTATTGCCGC1709940.29871895384462516No Hit
GCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAA1622700.2834785117627947No Hit
CAACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCT1540970.26920064230671953No Hit
CACATGTTAGGTGTAGCTGGTGTATTCGGCGGTTCCCTATTCAGTGCTAT1446610.2527163677211909No Hit
TGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTACGGAAACAATA1430270.24986184200343403No Hit
ACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCG1369540.2392525796509631No Hit
ACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCC1344410.23486247981698333No Hit
CAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGT1253070.21890578587206827No Hit
TAACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCT1248890.21817555836287467No Hit
GGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTACGGAAACAATATTAT1220250.21317227705586386No Hit
ACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTG1178880.20594512106176338No Hit
AAACGGATTCAATTTCAACCAATCTGTAGTTGATAGCCAAGGTCGCGTTA1160420.20272024072211883No Hit
GTAGTTGATAGCCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAA1124290.19640848954815582No Hit
GACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCT1101030.19234507044197316No Hit
TCACATGTTAGGTGTAGCTGGTGTATTCGGCGGTTCCCTATTCAGTGCTA1091280.1906417885724426No Hit
TCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGA1085740.1896739750793965No Hit
TAACCGGGGGATTCACCGCAAATACTAGTTTGGCTCATTATTGCCGCGAC944140.16493708146652183No Hit
CACAAGGATGAAATATCAGTAATTTTCTATTTACTGGTTGATCCCATCTT928560.1622153243868002No Hit
AAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCA901590.1575037846923141No Hit
ATCGAGTAGACCCTGTCGTTGTGAGAATTCTTAATTCATGAGTTGTAGGG876680.15315211788513397No Hit
AGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAA869550.15190653842567212No Hit
GTAGCTGGTGTATTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTT843350.14732951432498484No Hit
AGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAA820460.14333073258205617No Hit
TACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAAC788630.13777017238645023No Hit
TGCTCGTGAAGGTAATGAAATTATCCGATCAGCTTGCAAATGGAGTCCTG776610.1356703315585777No Hit
GTATTTATTATCGCCTTCATCGCTGCCCCTCCAGTAGATATTGATGGTAT762040.13312501701098176No Hit
GGTTCTTTACTTTACGGAAACAATATTATCTCTGGTGCCATTATTCCTAC761660.13305863269196413No Hit
CCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTG745600.13025302173558867No Hit
CTGCCCCTCCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCT735380.12846763294516791No Hit
TAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTAC730070.1275399994346851No Hit
TAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTC709150.12388536797718978No Hit
CTGCAACTGGATAACTAGCACCGAAAACCGTCTTTACATCGGATGGTTCG662820.11579172192433328No Hit
TGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCCTCCAGTA655460.11450596248230814No Hit
TATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCCTCCA648350.11326387693437355No Hit
AAACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCT646690.11297388227761244No Hit
GGTGTATTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAAC593430.10366959588056651No Hit
GGGTATGCGTCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTG591630.1033551438431147No Hit
TTCACATGTTAGGTGTAGCTGGTGTATTCGGCGGTTCCCTATTCAGTGCT589730.1030232222480267No Hit
GCTGCCCCTCCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTC575560.1005477859309756No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCCTT2629850.056.8242954
AACAAGC2612600.056.6895071
AGCCTTC2668200.056.0367245
CCTTCTA2759550.054.347597
CTTCTAT2856350.052.513728
GCCTTCT2899750.051.813316
TAGCCTG156150.047.5858461
GGTGTGC597850.043.816967
TGTGCTC496200.040.5699549
TTCTATT3742350.040.1878059
GTAGACC240850.037.2343946
CGAGTAG240300.037.043013
ACAAGCC4208300.036.1506772
CAAGCCT4325800.035.3310133
TAGACCC254300.035.2463767
CGACGCT267600.033.992438
GACGCTC260700.033.798959
CCGAGCC164000.032.409833
GGGTGTG336050.032.2276
AGTAGAC282400.031.6547575
Produced by FastQC (version 0.11.2)