FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l03n02_total_salt24h_rep1_shoot.35200000067278.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l03n02_total_salt24h_rep1_shoot.35200000067278.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68022847
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA3578300.5260438452392326No Hit
GCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1963370.288633905605274No Hit
CTCTAGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1928560.28351650732878025No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1908810.28061307107595773Illumina Single End PCR Primer 1 (100% over 50bp)
CTCTATAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1778130.2614018786952566No Hit
GCCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1719600.25279741672676537No Hit
CTCTCAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1605120.2359677771205313No Hit
CTCTACAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1559520.22926414708869802No Hit
CTCTGAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1553890.22843648399485544No Hit
CTCTTAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1493780.21959974712613836No Hit
TGACCAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT1491710.21929543760495648No Hit
GCCGCTGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1151240.16924313679490657No Hit
GCCGCCGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG951090.13981919927579628No Hit
TGACCGAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT883280.1298504897920547No Hit
CCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT789040.11599632105959928No Hit
GCCGTAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG741650.10902954414713044No Hit
CGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA693940.10201572421689437No Hit
CTCTGGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA688280.1011836508401361No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCAGT3065250.068.02889
ATTGCAG3101700.067.267648
CCGCGGA329000.061.844222
CGCGGAA417900.057.6008153
CGGAAGT505550.055.5777445
GCCGCGG340800.054.4352261
GAATTGC766400.049.884716
GCGGAAG564150.049.4463234
GAAGTAG2448600.049.3230977
AAGTAGG2489250.048.455728
AATCTGT891000.047.1549577
AGTAGGA2568900.047.0254449
TCTGTAA919400.045.6184739
ATCTGTA934450.044.9091768
CCGCTGA317750.042.1861082
GGAAGTA847800.042.159376
CGCTGAA336350.041.268253
TGACCGA237550.040.4368251
TAATTGC602950.040.3023386
CATACGC264000.040.2707679
Produced by FastQC (version 0.11.2)