FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l03n02_total_ctrl_1h_rep2_shoot.352000000673ba.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l03n02_total_ctrl_1h_rep2_shoot.352000000673ba.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76308581
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG4401300.576776548891664No Hit
GCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG4167160.5460932368798733No Hit
GCCGCTGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG3602400.4720832117163861No Hit
GCCGCCGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG2620050.34334932790848255No Hit
GGAGGGGCAGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA2472710.32404088342305826No Hit
GGGGGGGCAGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA1529150.2003903073495758No Hit
GGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA1468030.1923807232111943No Hit
CGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA1239290.16240506424827897No Hit
GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT1110600.14554064371869266No Hit
GCCGTGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG843410.11052623295406319No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGGA625400.076.07882
GCCGCGG629500.075.174381
CCGCTGA558950.068.973682
CGCGGAA781050.067.7388153
CGGAAGT873500.066.405915
CGCTGAA607200.066.118463
GCCGCTG623750.062.849791
GCGGAAG985700.059.5303574
GAAGTAG4079800.055.5483447
CATACGC386950.055.1468629
GGCCGCA403300.054.8894271
CCGAAGT545050.054.688235
AAGTAGG4143700.054.6732528
AGTAGGA4250550.053.3078279
AGCGATG909250.052.585549
CAGCGAT918300.051.9897088
GGAAGTA1383350.051.459976
GCAGCGA929750.051.4127667
CCGCCGA552550.051.0725982
CCGGCTC279550.050.1600232
Produced by FastQC (version 0.11.2)