FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l03n02_total_ctrl_025h_rep2_shoot.3520000006735e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l03n02_total_ctrl_025h_rep2_shoot.3520000006735e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76810347
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA6426570.8366802456965857No Hit
TGACCAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT5938040.7730781375066564No Hit
GCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG4670230.6080209480110798No Hit
GCCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG4056850.5281645192932145No Hit
CTCTAGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA4030350.524714463274069No Hit
TGACCGAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT3887710.5061440485355443No Hit
CTCTATAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA3401800.4428830402237344No Hit
CTCTCAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA3291200.4284839384985463No Hit
CTCTGAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA3261010.42455347845258395No Hit
GCCGCTGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG3101730.4038166889156223No Hit
CTCTACAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA3060380.39843329961782364No Hit
CTCTTAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA2917710.3798589791555036No Hit
GCCGCCGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG2674790.3482330316773598No Hit
TGGCCAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT2621760.3413290139152737No Hit
GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT2570430.33464632050158555No Hit
TCAAAAATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATGGA2485680.3236126507799789No Hit
GCCGTAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG2228100.2900781062738852No Hit
CCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT2117900.2757310808659672No Hit
ACCGGCTCGAACTCGAATTTGATCGCTTTCCATATTTCACAAGCTGCGGC1958490.25497736652589265No Hit
CGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA1883670.24523649138051673No Hit
TGGTTTATTCAAATTGCAAGGACTCCCAAGCACACGTATTAACTAGAAAG1878290.24453606491323363No Hit
GGAGGGGCAGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA1877880.24448268668803178No Hit
GCCGTGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1816010.23642778231427597No Hit
CTCTGGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1589360.2069200390410943No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1397980.18200412504320543Illumina Single End PCR Primer 1 (100% over 50bp)
GTCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1300250.1692805788261834No Hit
TCAAAGATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATGGA1282620.16698531514250287No Hit
TGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA1253220.16315770582314906No Hit
GCTGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1234310.16069579792420413No Hit
CTCCAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1175940.1530965613265619No Hit
CCCCCAGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG1154400.15029225164156595No Hit
TGACCTAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT1142220.1487065277806908No Hit
GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG1135370.14781472084744002No Hit
GGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA1098410.14300286913168092No Hit
CTCAAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1083170.14101876144368936No Hit
CCGGGGTGTAGTAAGTCAATTTATAATCCTTAACACCAGCTTTAAATCCA1024960.13344035537295518No Hit
CTTCAAAAATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATG1015150.13216318369190547No Hit
TGGCCGAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT969250.12618742628515922No Hit
GCTGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG944730.12299514803650086No Hit
CCCCCGGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG930190.12110217390373201No Hit
CTCGAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA913210.11889153423561541No Hit
CCGCAAAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT910900.11859079350338046No Hit
CCGCATAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT898770.11701157918216408No Hit
TCAAGAATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATGGA895930.11664183732954625No Hit
ACTGGAGGGGCAGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAA891480.11606248829991615No Hit
CCGCTGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT881980.11482567576475082No Hit
GGGGGGGCAGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA877390.11422810002407618No Hit
GGGTATGCGTCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTG865800.11271918873117445No Hit
AGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA854620.11126365566347461No Hit
CCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT843060.10975865009436815No Hit
CCCCCCGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG812500.10578001945493098No Hit
CCGCCGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT807050.10507047963212561No Hit
GTCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG795220.1035303225488618No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCGA555100.075.420861
ACCGAAT617900.071.83513
CGAATCT789650.071.687445
CCGAATC799800.070.907514
CCGCGGA717950.070.4644852
GACCGAA605200.070.442142
AATCTGT2943500.068.831177
TTGCAGT6725500.068.4409949
ATTGCAG6785500.067.914228
CGGAAGT1149500.066.2063755
GAATCTG904500.065.347886
CGCGGAA946100.064.899923
GCCGCGG671600.064.395311
CCCGGAC214000.064.374913
CATACGC1213700.064.065449
ATCTGTA3197450.063.5143558
TCTGTAA3195650.063.4624259
GAATTGC1466450.063.4328426
TAATTGC855000.062.2869456
CTCTACA524500.062.0407681
Produced by FastQC (version 0.11.2)