FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l03n02_total_ctrl_025h_rep1_shoot.352000000672e2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l03n02_total_ctrl_025h_rep1_shoot.352000000672e2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35780480
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACCAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT2740840.7660154363496521No Hit
GCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG2509780.7014383261487828No Hit
GCCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG2020070.5645731974529129No Hit
CTCTAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1897570.530336652834171No Hit
GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT1798630.5026847040621031No Hit
GCCGCTGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1628760.4552090972507915No Hit
TGACCGAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT1526180.4265398340100524No Hit
TCAAAAATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATGGA1376310.3846538671364945No Hit
GCCGCCGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1342990.37534152700019674No Hit
CTCTAGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1215560.3397271361367986No Hit
TGGCCAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT1159680.32410968215071456No Hit
GCCGTAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1130280.31589291144221654No Hit
ACCGGCTCGAACTCGAATTTGATCGCTTTCCATATTTCACAAGCTGCGGC1096750.30652188008657233No Hit
CTCTATAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA997900.27889508469422436No Hit
CCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT978770.27354859409376286No Hit
CCCCCAGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG927100.25910775931457597No Hit
CTCTGAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA892490.24943488740229308No Hit
GCCGTGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG850860.23780005187185863No Hit
CGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA844680.2360728531310927No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC840190.23481797896506695Illumina Single End PCR Primer 1 (100% over 50bp)
CTCTCAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA838980.23447980574883287No Hit
CTCTACAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA837660.23411088951294112No Hit
CTCTTAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA816840.22829207433773946No Hit
TCAAAGATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATGGA792480.22148389289355536No Hit
CCCCCGGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG737130.2060145643658218No Hit
TGGTTTATTCAAATTGCAAGGACTCCCAAGCACACGTATTAACTAGAAAG678100.18951674208954156No Hit
GGGTATGCGTCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTG620490.17341578424884183No Hit
CCCCCCGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG577590.16142600658236No Hit
GTCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG577210.16131980342354268No Hit
TGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA576190.16103473178671723No Hit
TGACCTAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT571750.15979383172053588No Hit
GCTGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG560380.15661612141592288No Hit
CTTCAAAAATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATG544960.1523065090239147No Hit
GGAGGGGCAGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA522810.14611598279285243No Hit
TCAAGAATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATGGA513110.14340500742304182No Hit
CCCCCTGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG504950.1411244343284383No Hit
GGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA498820.13941120968751677No Hit
CTCTGGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA463760.12961257087663441No Hit
GCTGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG436670.12204140358094692No Hit
CCGCAAAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT418310.11691011411808898No Hit
CCGCATAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT404790.11313151752016742No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT394040.11012708605362476No Hit
AGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA393340.10993144865580337No Hit
CCGCTGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT388950.10870452268946643No Hit
ATGGTCATTGAGGGCCTCCTAAAAGGATCTACTAAATTCATCGAGTTGTT386340.10797507467758957No Hit
CCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT382950.10702763070814031No Hit
TGGCCGAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT382780.10698011876866939No Hit
GTCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG368030.10285775931457598No Hit
TCATCCATAGATCCTTTACTCATATTTAAAAAATTGGAATCCTTAATCCA366530.10243853631924445No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCGA228500.072.606791
CCCGGAC138900.071.45383
CCGCGGA354750.070.245922
TTGCAGT1773750.070.197159
CGAATCT319250.069.668315
ATTGCAG1806450.068.942248
CGGAAGT534800.067.556025
CATACGC773450.067.449319
CGCGGAA446150.067.035223
ACCGAAT265250.066.83193
AATCTGT1365200.066.7489557
CCGAATC336600.066.310364
GACCGAA255850.066.238552
GCCGCGG331150.066.1023251
CGGACAT178750.065.761425
CCGGACA167200.064.474394
ACATACG834350.062.486278
GAATCTG378400.062.373926
GGACATA226100.062.3307276
TGCTATA1037050.062.095519
Produced by FastQC (version 0.11.2)