FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l03n01_total_salt24h_rep1_shoot.3510000006727b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l03n01_total_salt24h_rep1_shoot.3510000006727b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68022847
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTT9009611.3244976353312585No Hit
TTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG7736911.1373987331050697No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG1896470.27879897470330817TruSeq Adapter, Index 4 (100% over 49bp)
AGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT1356060.19935360835455768No Hit
CCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTA1044810.15359692310437992No Hit
GCCCCTCCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTT1040860.1530162358538154No Hit
TGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTACGGAAACAATA882730.12976963460526725No Hit
ACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTG845910.12435674737342292No Hit
AGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTCT808310.11882919278577092No Hit
ATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATA746400.10972783894211309No Hit
GGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTACGGAAACAATATTAT705200.10367105040457951No Hit
TTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAA684870.10068234868205383No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTGC1076200.056.2253347
AGCCTTC1625000.055.69585
AAGCCTT1641900.055.3119624
CCTTCTA1674000.054.074037
AACAAGC1787950.050.7695051
GCCTTCT1795400.050.5049676
CTTCTAT1853700.048.890968
TGTGCTC1100600.046.8392529
CAAGCCT2076050.044.3347783
GGGTGTG620450.043.989416
ACAAGCC2095650.043.746392
TTCTATT2258200.040.3584379
CGGTGTG337600.033.303146
TCTATTA1828950.028.65725510-11
CTAGTTA2756800.028.4921263
TAATACG2802250.027.8886748
TTCTAGT2823550.027.4956471
AATACGT2841050.027.4576459
TAGTTAA2874550.027.2839784
CTATTAT1846750.027.15804310-11
Produced by FastQC (version 0.11.2)