FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l03n01_total_ctrl_1h_rep2_shoot.351000000673bd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l03n01_total_ctrl_1h_rep2_shoot.351000000673bd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76308581
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCCTCCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTT3798530.4977854325452599No Hit
AGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTCT3484250.45660002510071573No Hit
CCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTA2702430.3541449683096584No Hit
GCAACTGGATAACTAGCACCGAAAACCGTCTTTACATCGGATGGTTCGGT2456510.3219179242764323No Hit
ACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCG2235180.292913322552807No Hit
AAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTC2187090.2866112790119895No Hit
TACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTC2120530.27788879995029653No Hit
CAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGT1355770.17766940260624162No Hit
CCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTG1044910.13693217542598518No Hit
TTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAA1036810.1358706958526722No Hit
ACTTAACCGGGGGATTCACCGCAAATACTAGTTTGGCTCATTATTGCCGC944630.12379079621464852No Hit
TGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCCTCCAGTA895200.11731314988022119No Hit
CTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCC802840.10520966180723502No Hit
TACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAAC777930.10194528450214531No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAGCG185750.042.169712
ACGCAGC250700.033.623371
CAGCGGA290550.031.5999324
GCGGAAA293700.029.8386976
ACCGGGG327850.028.628546
AGCGGAA323950.028.313385
TGACTGC801900.027.1407786
TAACCGG348150.026.5628554
CCATGAC842350.026.5131823
ATGACTG837350.026.0768385
GCAGCGG363850.025.5992363
CGGGGGA370650.025.4252438
CATGACT877700.025.2290784
CCGGGGG383650.025.170377
TTAACCG373350.024.7697473
GCCGAGC202750.024.6938342
TACCATG896300.024.6779041
AACCGGG385850.024.5467555
CCGAGCC209350.024.0947993
GACTGCA910600.023.9530967
Produced by FastQC (version 0.11.2)