FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l03n01_total_ctrl_025h_rep2_shoot.35100000067351.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l03n01_total_ctrl_025h_rep2_shoot.35100000067351.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76810347
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG19462392.533823991187021No Hit
AACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTT13498201.7573413644388303No Hit
AGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTCT4347530.5660083790534106No Hit
AGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT3500900.45578494782740664No Hit
GCCCCTCCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTT2787360.3628886092651033No Hit
ATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATA2462240.3205609785879499No Hit
AAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTC2453440.31941529960800724No Hit
CCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTA2416250.3145735040098179No Hit
TATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA2363020.3076434480890966No Hit
TTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAA2261620.2944421016611212No Hit
GCAACTGGATAACTAGCACCGAAAACCGTCTTTACATCGGATGGTTCGGT2098510.2732066813862981No Hit
GGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTACGGAAACAATATTAT1936020.2520519794032437No Hit
ATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAAC1876500.24430302339344986No Hit
CAACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCT1835620.23898082376844357No Hit
TGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTACGGAAACAATA1817470.23661786087231193No Hit
ACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCG1603590.20877265402797882No Hit
TCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGA1593910.2075124071500419No Hit
CACATGTTAGGTGTAGCTGGTGTATTCGGCGGTTCCCTATTCAGTGCTAT1556800.20268102681530653No Hit
CAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGT1530040.19919712118993552No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG1477120.19230742441509865TruSeq Adapter, Index 6 (100% over 49bp)
ACTTAACCGGGGGATTCACCGCAAATACTAGTTTGGCTCATTATTGCCGC1466090.1908714199663751No Hit
GCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAA1362200.17734589846339321No Hit
AAACGGATTCAATTTCAACCAATCTGTAGTTGATAGCCAAGGTCGCGTTA1347420.1754216785402623No Hit
TACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTC1319670.17180888402964772No Hit
TCACATGTTAGGTGTAGCTGGTGTATTCGGCGGTTCCCTATTCAGTGCTA1262950.16442446224074472No Hit
GTAGCTGGTGTATTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTT1250450.16279707732605347No Hit
GTAGTTGATAGCCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAA1202410.15654271162191208No Hit
ACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCC1156010.1505018588185782No Hit
GGTTCTTTACTTTACGGAAACAATATTATCTCTGGTGCCATTATTCCTAC1154850.15035083749849482No Hit
ACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTG1124990.14646334041428039No Hit
CTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCC1098460.14300937867133967No Hit
TAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTAC1086070.14139631474389772No Hit
AGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAA1082250.14089898591396807No Hit
TAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTC1056790.13758432831972495No Hit
TAACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCT1021960.13304978299342926No Hit
GTATTTATTATCGCCTTCATCGCTGCCCCTCCAGTAGATATTGATGGTAT1005360.13088861582671926No Hit
AAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCA997510.12986661810029318No Hit
ATCGAGTAGACCCTGTCGTTGTGAGAATTCTTAATTCATGAGTTGTAGGG991330.12906203899846982No Hit
AGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAA948000.12342087193018408No Hit
TACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAAC942320.1226813882249484No Hit
TAACCGGGGGATTCACCGCAAATACTAGTTTGGCTCATTATTGCCGCGAC886890.11546491255924153No Hit
CACAAGGATGAAATATCAGTAATTTTCTATTTACTGGTTGATCCCATCTT884600.1151667756428701No Hit
TGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCCTCCAGTA851330.11083532795392788No Hit
CAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTC848970.11052807768203417No Hit
GGTGTATTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAAC834490.10864291499685583No Hit
TATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCCTCCA781180.10170244381268059No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTGC1877250.056.5388037
AAGCCTT2584050.053.2343864
AGCCTTC2592450.053.0593035
TGTGCTC1518150.052.7541969
AACAAGC2644250.051.7629051
GGGTGTG877200.051.6743056
CCTTCTA2702650.050.858917
CTTCTAT2848500.048.311518
GCCTTCT2908600.047.336126
TAGCCTG252000.042.7076721
CGGTGTG439000.041.2996146
CGACGCT556300.037.3472028
GACGCTC546750.037.0352179
TTCTATT3739650.036.892989
CGAGTAG274500.036.5968483
TGGGTGT516150.035.5405965
AGGGTGT395050.035.5298845
GTAGACC291100.034.608826
AGTGTGC375700.033.238227
TGGTGTG1222000.033.1096656
Produced by FastQC (version 0.11.2)