FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l03n01_total_ctrl_025h_rep1_shoot.351000000672e5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l03n01_total_ctrl_025h_rep1_shoot.351000000672e5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35780480
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG4038741.1287551201101829No Hit
AACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTT3639951.0173004945713418No Hit
AGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTCT2188620.6116798880283327No Hit
AAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTC1392480.3891730910261684No Hit
TTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAA1295410.36204377358828055No Hit
GCAACTGGATAACTAGCACCGAAAACCGTCTTTACATCGGATGGTTCGGT1003730.28052446473607956No Hit
AGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT915990.2560027143291538No Hit
CAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGT873400.24409957608170713No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG866840.2422661741821239TruSeq Adapter, Index 7 (100% over 49bp)
ACTTAACCGGGGGATTCACCGCAAATACTAGTTTGGCTCATTATTGCCGC865460.2418804890264189No Hit
ACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCG828610.23158157744110752No Hit
TATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA714890.19979888475503962No Hit
TACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTC696400.19463126263258626No Hit
ATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATA650570.1818226027152235No Hit
AGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAA637400.17814182481621263No Hit
ACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCC627300.175319056647647No Hit
GCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAA622860.17407815658146564No Hit
TAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTC599060.16742648505553867No Hit
ATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAAC589490.16475184234532347No Hit
GCCCCTCCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTT572320.15995313645876189No Hit
AAACGGATTCAATTTCAACCAATCTGTAGTTGATAGCCAAGGTCGCGTTA547120.15291019013719212No Hit
TACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAAC519480.14518530774321642No Hit
TAACCGGGGGATTCACCGCAAATACTAGTTTGGCTCATTATTGCCGCGAC510890.14278455738995116No Hit
CACATGTTAGGTGTAGCTGGTGTATTCGGCGGTTCCCTATTCAGTGCTAT500960.14000930116085641No Hit
CAACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCT491540.1373765807501744No Hit
CCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTA489120.13670023431770617No Hit
CTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCC483660.1351742626146994No Hit
CAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTC474420.1325918489634572No Hit
GTAGTTGATAGCCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAA462370.12922409090096051No Hit
GGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTACGGAAACAATATTAT449450.1256131835011716No Hit
TCACATGTTAGGTGTAGCTGGTGTATTCGGCGGTTCCCTATTCAGTGCTA448660.1253923927236303No Hit
TGCACTTTTACCCAATTTGGGAAGCTGCATCTGTTGATGAGTGGTTATAC422050.11795537678644893No Hit
TATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCCTCCA414200.11576144311088057No Hit
GCTAATGAGGGTTACAGATTTGGTCAAGAGGAAGAAACTTATAATATTGT381280.10656089577333787No Hit
GGGTATGCGTCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTG380750.10641277031498739No Hit
TCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGA370660.10359279696639061No Hit
TGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTACGGAAACAATA363250.10152183536945283No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTGC996350.052.2068447
TGTGCTC813650.050.2399649
GGGTGTG423750.049.4439246
AGCCTTC788200.049.361245
CGACGCT448600.047.721488
AAGCCTT822200.047.238234
GACGCTC450500.046.8454179
CCTTCTA838600.046.428617
TAGCCTG134400.045.7826771
CTTCTAT908900.043.0047658
AACAAGC900550.042.0356371
CGGTGTG230900.041.060316
GCCTTCT952900.040.879456
GCGACGC244000.038.563847
TGGGTGT250850.035.3710825
CCGACGC339800.034.5549857
TGTGGTG483100.033.9890824
CGAGTAG101700.032.645843
TAGGGTG83400.032.57674
TGCGACG181850.032.1275376
Produced by FastQC (version 0.11.2)