FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l02n02_w9_hiseq.352000000674e0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l02n02_w9_hiseq.352000000674e0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17163291
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG122250.021.599525
CTATACT143800.017.898874
TAGACTG59300.017.7793735
TTATACT200950.017.7238664
ACTGTGC79550.017.1992178
TACACTG84900.016.7815365
TAATACT204200.016.767364
TTACACT112450.016.2164964
ATACTGT121100.016.118886
ACTGCGC31050.015.7591198
CTGTGCA106450.015.7538939
TAAGGCT73250.015.4944274
GTATTAG112000.015.3902141
AGACTGT60700.015.1010046
GACTGTG50450.015.0636497
TTAACCT106250.015.0621224
CCAGGAC53600.015.0608353
CTGTGCG39800.015.0399489
ACTGTGT90900.014.9994158
GTATAGG77300.014.8659251
Produced by FastQC (version 0.11.2)