FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l02n02_w8_hiseq.35200000067469.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l02n02_w8_hiseq.35200000067469.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23887818
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG168050.021.280535
ACTGTGC103250.017.5761998
TAATACT278900.017.3501954
TTATACT272100.017.0333444
CTATACT192100.016.859154
ATACTGT166750.016.6908956
TAGACTG75450.016.5547455
TACACTG122750.016.4047785
CTACACT115200.015.994034
CTAGACT88900.015.81133654
GTATTGG228050.015.7831331
CTGTGCG53750.015.7323689
TAAGGCT102450.015.2033934
TATACAG145550.015.1728355
AGTACTG138500.015.1564855
CTGTACT136350.015.1151774
GTATTAC127600.015.0343541
TCTATAC162350.014.9754523
CTACCCT85700.014.9610334
GACTGTG68200.014.9043717
Produced by FastQC (version 0.11.2)