FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l02n02_w5_hiseq.352000000673fe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l02n02_w5_hiseq.352000000673fe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19992515
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG127350.018.9072155
CTATACT161250.017.0808644
TAATACT202450.016.5133974
GTATAGG108300.016.4856951
ATACTGT133900.015.8906686
TTATACT203500.015.6347884
ACTGTGC88650.015.486848
TACACTG89950.015.1002255
GTATTGG136500.014.8192011
TAGACAG64600.014.7033415
CCTATAC116100.014.5605443
ACTGTGT105400.014.2426298
CTGTGCG44550.014.1823319
TTACACT111850.013.8408634
CTACACT88600.013.7746634
GTATTAC100300.013.729191
TAGGACT80200.013.6186814
TAGTACT218700.013.5058894
TATACAG115200.013.4807435
CTGTGCA141050.013.4719559
Produced by FastQC (version 0.11.2)