FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l02n02_w4_hiseq.35200000067391.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l02n02_w4_hiseq.35200000067391.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13781718
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG92200.020.4496785
TAATACT118200.020.1686534
CTATACT92800.018.2688354
TTATACT120550.017.9633884
TATACAG89200.017.6766575
TATACCG44750.017.6174055
GTATTAT109400.017.4055461
TCTATAC73550.016.528273
GTATAGG61200.016.4492111
ATAATAC108350.016.2597963
TAGACTG49800.015.92627055
GTATTAC70550.015.8172681
GTATTGG110750.015.7784641
GTATAAT108400.015.68246651
GTATTAG69100.015.5994211
ACTGTGC68150.015.5445458
TATACTT120300.015.4755955
ATTATAC101500.015.4389593
GTATATA196450.015.4215961
GTAATAC69850.015.2962943
Produced by FastQC (version 0.11.2)