FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l02n02_w3_hiseq.35200000067327.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l02n02_w3_hiseq.35200000067327.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17841146
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG111700.022.4026641
CTATACT141800.021.4324974
TATACTG103750.020.0954555
TAATACT129800.019.901844
CCTATAC125750.019.2580783
TATAGGG105250.018.857982
TATACCG55750.017.8894045
TATACTA180600.017.8292645
GTATAAT123800.017.6049291
TTATACT132050.017.369124
GTATTAG77750.016.9169311
GTATTAT121450.016.7726611
ATAATAC117300.016.5163063
GTATATA230100.016.3849871
TATACAG101300.016.174515
TAGACTG59950.016.0023465
GTATAAG80950.015.896251
CTAATAC77300.015.7871743
CCCTATA115300.015.7728612
GTATAGA89950.015.519881
Produced by FastQC (version 0.11.2)