FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l02n02_w2_hiseq.352000000672bb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l02n02_w2_hiseq.352000000672bb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16033677
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG81950.018.3675211
CTATACT104850.017.617844
TATACTG89950.017.3173775
TAATACT125600.016.106144
GTATATA211200.015.9851241
CCTATAC83850.015.9132853
ATAATAC111550.015.7077283
TTATACT122300.015.4923724
GTATTAT118550.015.3804841
TACACTG69050.015.1998075
GTATAAT121250.015.1946341
TATACAG91650.015.0789125
TATACTA154750.014.5157665
GTATTAG75800.014.4078421
TCTATAC78000.014.3672613
GTATTGG105200.014.2630251
GTAATAC69000.014.2455033
GTATAAG72550.014.1369791
TATACTT127700.014.0203855
ATTATAC108550.013.9108573
Produced by FastQC (version 0.11.2)