FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l02n02_w1_hiseq.3520000006725f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l02n02_w1_hiseq.3520000006725f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13964582
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG88950.019.38075
TATACAG83850.017.840885
TTATACT113800.017.0673644
CTATACT92650.016.8630394
ATTATAC100450.016.6876873
GTATATA195500.016.3951681
TAATACT114200.016.3838334
GTATTAT103650.016.2178341
TATACCG43800.016.0470925
GTATAAT103250.015.7287771
ATAATAC101550.015.5249273
CCTATAC62900.015.4765643
TACACTG68550.015.3799265
GTATTAG66750.014.8680471
ACTGTGC61750.014.847628
GTATTAC69200.014.8219911
GTATTGG110450.014.66045951
GTATAGG64100.014.4455971
TATACTA131050.014.0968265
TAGACTG45100.014.0050235
Produced by FastQC (version 0.11.2)