FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l02n02_w10_hiseq.35200000067532.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l02n02_w10_hiseq.35200000067532.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45758246
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG248500.018.5922551
CTATACT334000.015.8953284
TATACTG263000.014.6266775
CCTATAC265050.014.5293523
GTATATA594250.013.3601921
TATAGGG241600.013.3449532
TATACTA458800.013.2496065
GTATTAT334250.013.0411791
TAATACT361450.012.6123824
CCCTATA249050.012.4500422
GTATTGG276050.012.4192411
TAGACTG144400.012.3991135
ACTGTGC206300.012.36570558
TTATACT356200.012.2783454
CGCCGTA206300.012.27649494-95
TATACCG135950.012.2615215
GTATAAG205150.012.1747431
GTATAAT332350.012.1727721
TCTATAC214700.011.8988083
GTATTAC200450.011.8679921
Produced by FastQC (version 0.11.2)