FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l02n01_w9_hiseq.351000000674e3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l02n01_w9_hiseq.351000000674e3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17163291
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG123900.019.9350515
CTATACT138500.018.0046524
TAGACTG55150.017.4837995
TTATACT198550.017.2002284
TACACTG84600.016.7875045
TAATACT214250.016.7600844
ATACTGT127750.016.4712146
ACTGTGC78600.015.9535698
TAGGACT73750.015.90784
CTAGACT65900.015.8566874
TAACACT98050.015.8407354
CTGTGCA104900.015.7572429
CAGGACT51250.015.662754
TAAGACT86800.015.5955364
TCTATAC119450.015.5874063
CTGTGCG36850.015.4675049
TTACACT111850.015.4150824
TAGACAG60150.015.319745
CTACACT81550.015.3181734
GACTGTG51200.015.2142437
Produced by FastQC (version 0.11.2)