FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l02n01_w8_hiseq.3510000006746c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l02n01_w8_hiseq.3510000006746c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23887818
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG173900.021.140755
TACACTG121600.018.2806385
CTACACT118700.017.7666264
TTATACT273150.017.6844864
ACTGTGC110200.017.4992688
TAATACT285900.016.9456674
CTGTGCG52000.016.898339
ATACTGT173700.016.8718816
CTATACT194150.016.6602844
TATACAG149050.015.4557055
GTATTGG241100.015.3917311
TGTACTG182400.015.3640685
CTAGACT88650.015.2699924
CTGTGCA144450.015.2572369
TAGACTG72950.015.105735
AGGACTG81400.015.0547795
TAGGACT102850.015.0090014
TTACACT154900.014.9024474
TAGACAG82350.014.6503915
ACAGTGC111950.014.6376198
Produced by FastQC (version 0.11.2)