FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l02n01_w5_hiseq.351000000673f1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l02n01_w5_hiseq.351000000673f1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19992515
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG126650.020.1395055
CTATACT167900.017.4262244
TTATACT204300.017.018294
GTATAGG114600.016.9166741
TAATACT206300.016.6691174
TAGACTG65250.016.0148145
TACACTG88450.015.3047695
CTACACT84750.015.0199434
CTAGACT77700.014.9767734
ACTGTGC88150.014.6023388
GTATTAG109000.014.5599341
ATACTGT135500.014.5473986
TAGACAG60700.014.4764745
GTATAGA125100.014.2054081
TAGGACT81550.014.1532294
CCTATAC114450.014.1102683
GTATTAT180950.014.101171
CAGGACT63100.013.9256544
TCTATAC123900.013.7624363
TTACACT113550.013.4692054
Produced by FastQC (version 0.11.2)