FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l02n01_w4_hiseq.35100000067394.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l02n01_w4_hiseq.35100000067394.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13781718
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG90600.021.7045545
TAATACT120250.019.9864774
TTATACT120400.018.3835874
GTATTAG69950.017.8659611
CTATACT95600.017.8364074
CTACACT66450.017.4407714
CCTATAC61700.017.0128213
TATACAG92250.016.888315
ATAATAC109200.016.7023583
GTATATA205000.016.6660771
CTAATAC68400.016.387973
ATTATAC107250.016.3860283
GTATAGG60650.016.1396751
TAGACTG49750.016.1351245
GTATTGG114650.015.9153251
GTATAAG66000.015.839341
GTATTAT113450.015.8323611
TTACACT78200.015.792014
GTATAAT111800.015.5984931
GTATAGA78050.015.5247871
Produced by FastQC (version 0.11.2)