FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l02n01_w3_hiseq.3510000006732a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l02n01_w3_hiseq.3510000006732a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17841146
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG116250.022.644461
CTATACT145100.020.5900784
TATACTG105350.020.063485
CCTATAC118300.019.432693
TATAGGG109150.018.626242
TAATACT136300.018.5740264
GTATTAT120450.018.4227831
TTATACT135250.017.6295474
TATACCG56250.017.5639275
ATTATAC117400.017.4373913
ATAATAC122450.016.9509853
CCCTATA113950.016.8828532
GTATAAT127400.016.671831
TATACTA191150.016.5493435
GTATTGG115050.016.5203231
GTATAGA93200.016.4166721
GTATATA235550.016.1380861
GTAATAC78700.016.053853
TCTATAC90650.015.7190293
TAGACTG61250.015.5872965
Produced by FastQC (version 0.11.2)