FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l02n01_w2_hiseq.351000000672be.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l02n01_w2_hiseq.351000000672be.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16033677
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT108600.018.456894
TATACTG98200.017.7997935
CCTATAC82450.016.9943493
GTATAGG80300.016.9827331
GTATTAT118700.016.852821
TAATACT129200.016.506684
TATACAG93100.015.4076325
GTATAGA84300.015.3314251
GTATTAC72450.015.0189051
GTATATA222650.015.0028511
TTATACT120850.014.97456554
ATAATAC114000.014.9576313
TCTATAC81400.014.6461173
TACACTG70450.014.6304395
GTATTAG75550.014.2768551
ATTATAC114800.014.232783
GTATAAT114600.014.0972851
TATACTA153800.013.9900885
CTAATAC76900.013.8355023
TTACACT77700.013.7542674
Produced by FastQC (version 0.11.2)