FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l02n01_w1_hiseq.35100000067252.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l02n01_w1_hiseq.35100000067252.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13964582
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG89000.018.1456875
TTATACT109450.017.9228864
CTATACT91450.017.347484
TAATACT116150.016.6436614
GTATTAT105500.016.5745491
ATAATAC105200.016.299043
GTATATA192200.015.7976971
TATACAG85100.015.5167165
GTATAGG61200.015.2177691
ATTATAC101800.014.837133
GTATTAG67100.014.729471
CCTATAC62450.014.6029093
TAGACAG47650.014.354375
TCTATAC70350.014.3133843
GTATAAG62750.014.236081
TATACTT120450.014.2359015
TATACCG46050.014.2342315
TATACTA130500.014.2315045
CTACACT63800.014.070674
ACTATAC76650.014.0664423
Produced by FastQC (version 0.11.2)