FastQCFastQC Report
Tue 23 Aug 2016
C841RACXX_l02n01_w10_hiseq.35100000067535.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC841RACXX_l02n01_w10_hiseq.35100000067535.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45758246
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG256550.016.9104791
CTATACT342950.016.398244
CCTATAC267100.015.9334733
TATACTG261300.015.3967265
GTATTAT334750.013.8259671
ACTGTGC200150.013.3131478
GTATATA586300.013.2836121
TAGACTG148200.013.2048475
TTATACT358100.013.0384364
TATAGGG247650.012.9475412
TATACCG139950.012.897195
TAATACT368450.012.8010914
TACACTG199900.012.6885755
GTATTAG209300.012.6457081
TATACTA469100.012.5961935
ATACTGT294350.012.5221036
CCCTATA258400.012.44566252
ATTATAC323850.012.4373623
GTATTGG274150.012.3929581
GTATAAT336450.012.3437771
Produced by FastQC (version 0.11.2)