FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l08n02_tsr35.35200000055e81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l08n02_tsr35.35200000055e81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9376903
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGC22950.046.349439
GAGCGGC27700.042.515869
GGGCGCC32150.037.0914242-43
GGCGCCC9700.035.99872244-45
GGGGCGC27850.034.3740442-43
TCGGGGG47350.033.7618438-39
AGGGGCG26600.029.19246710-11
AGAGCGG41800.029.1915518
GGGCGGG27850.028.6520612-13
GATCGGG44850.028.6118951
GGGGGCG37300.028.33998140-41
GGGGCCC13000.028.13965844-45
GTAGGGG26150.028.06638718-19
AAGGGGC24000.027.7010279
GCGCCCG9900.027.11344544-45
CGGGTAG16750.026.79930514-15
CTCGGGG31700.026.7405836-37
ATCGGGA49850.025.5458222
TCGGGAG43900.025.3237133
GGGCCCG8500.024.31317944-45
Produced by FastQC (version 0.11.2)