FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l08n02_tsr32.35200000055e67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l08n02_tsr32.35200000055e67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7213588
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGC16150.041.459489
GGCGCCC5150.036.43655444-45
GGGCGCC20300.034.1673942-43
GAGCGGC21750.030.5665259
AAGGGGC17350.030.1072049
TCGGGGG34350.029.94153838-39
GGGGCGC20800.029.46329742-43
GATCGGG34800.026.906981
GGGCGGG20800.026.60352712-13
AGGGGCG20900.026.47348610-11
GGGGCCC9000.026.39215944-45
CTCGGGG23900.026.03021436-37
GGGCCCG5350.025.30687344-45
AGAGCGG28850.023.3692368
GGGGGCG27350.023.18875340-41
ATCGGGA39850.023.1321352
GTAGGGG19700.021.70146618-19
TCGGGAG37650.020.9475983
AGGGTAG22500.019.84435828-29
AGAGGGG39050.019.6968148
Produced by FastQC (version 0.11.2)