FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l08n02_tsr29.35200000055e4e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l08n02_tsr29.35200000055e4e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10663706
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGC20000.029.6797059
GGCGCCC7600.029.37989844-45
GGGCGCC28500.024.4213742-43
GGGCGGG21050.021.77400412-13
TCGGGGG48400.021.2508638-39
GTAGGGG23000.020.65353418-19
GAGCGGC30150.020.6330579
GGGGGCG29900.020.33836440-41
GGGGCCC10700.019.75796744-45
GATCGGG42950.018.9235151
GTGTGCG20450.018.5731078
GGGGCGC31350.018.48840742-43
CGGGTAG14800.017.49188414-15
TAGGGGA28750.017.10112818-19
CTCGGGG30300.016.92688436-37
ATCGGGA48650.016.4084222
GGGCCCG5650.016.3965444-45
GGGGGGC34850.016.22263340-41
AGGGTAG28900.014.95678528-29
CGGGGTG9000.014.77805714-15