FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l08n02_tsr26.35200000055e24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l08n02_tsr26.35200000055e24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13852021
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGC40050.048.8519139
GAGCGGC51500.042.416769
GGGCGCC61100.039.03280642-43
GATCGGG70750.038.158251
TCGGGGG82800.037.0930838-39
GGCGCCC18950.036.3509644-45
AAGGGGC38900.035.7688459
GGGGCGC54800.034.850842-43
AGAGCGG67300.033.652688
ATCGGGA82250.032.870862
AGGGGCG47000.032.4362710-11
TCGGGAG74450.032.162543
GTAGGGG49250.030.76760318-19
GGGCGGG52550.030.00739712-13
CTCGGGG57500.029.98045536-37
CGGGAGA93600.029.4330124
GGGGCCC25350.028.57912644-45
GGGGGCG72150.028.28090740-41
TAGGGGA55600.027.59541518-19
GCGCCCG17750.026.09536444-45
Produced by FastQC (version 0.11.2)