FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l08n02_tsr23.35200000055dfc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l08n02_tsr23.35200000055dfc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11767977
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGC29850.042.3173759
GGCGCCC11200.035.4186344-45
GAGCGGC40300.033.347469
GGGCGCC41950.032.50271642-43
GATCGGG51550.030.24151
TCGGGGG69350.028.60011338-39
GGGGCCC18050.028.03081144-45
GTAGGGG30500.027.8782618-19
GGGCGGG38650.027.34350812-13
GGGCCCG11550.027.1472144-45
AAGGGGC35350.027.0015079
AGAGCGG51650.026.5665478
ATCGGGA61400.025.5372682
GGGGCGC44150.025.12614842-43
CGGGTAG20050.024.99389514-15
AGGGTAG34850.024.19283928-29
GGGGGCG50250.023.96688740-41
TAGGGGA35950.023.45373218-19
AGGGGCG45350.022.62083410-11
CGGGAGA66900.022.3653034