FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l08n02_tsr20.35200000055dd2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l08n02_tsr20.35200000055dd2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13051086
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGC44800.050.2436149
GAGCGGC51850.047.6250159
GATCGGG76850.043.724261
GGGCGCC69550.039.653142-43
GGCGCCC19500.038.98079744-45
AGAGCGG66550.038.452818
AAGGGGC42900.038.0786679
ATCGGGA89550.037.1947022
CGGGAGA86650.035.6303634
TCGGGAG81100.035.616653
TCGGGGG107600.035.40958438-39
GGGGCGC63200.034.1655842-43
GTAGGGG52800.034.05341318-19
GGGCGGG62050.031.843112-13
CGGGTAG32250.030.71019714-15
AGGGGCG59250.030.69941710-11
TAGGGGA58500.030.4511818-19
GGGGGCG81300.029.51024840-41
CTCGGGG68800.029.40475336-37
GGGGCCC28600.028.98643144-45
Produced by FastQC (version 0.11.2)