FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l08n02_tsr14.35200000055d9f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l08n02_tsr14.35200000055d9f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12388486
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGC33500.039.6913459
GGCGCCC12800.037.344-45
GAGCGGC40550.035.367049
GGGCGCC47400.034.3778842-43
GATCGGG59800.032.1104971
TCGGGGG76050.030.98377438-39
GTAGGGG35800.029.32513818-19
GGGGCGC45400.029.0382542-43
AAGGGGC35650.028.772459
ATCGGGA70450.027.450732
AGAGCGG53650.027.4349698
GGGGCCC20700.026.50723344-45
GGGCGGG45000.026.33475112-13
CTCGGGG47600.025.78988536-37
CGGGTAG20250.025.68598414-15
TAGGGGA42100.025.10607118-19
AGGGGCG44800.024.96544810-11
TCGGGAG66800.024.8926243
CGGGAGA73050.024.7083764
AGGGTAG42100.024.42643728-29