FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l08n02_tsr08.35200000055d5c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l08n02_tsr08.35200000055d5c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10139238
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGC27050.048.101749
GATCGGG43900.039.1934781
GGCGCCC11250.038.0060644-45
GAGCGGC39550.036.981269
TCGGGGG55600.035.3739138-39
AAGGGGC24500.034.5009779
GGGGCGC35250.034.03083442-43
AGAGCGG43650.032.957358
GGGCGCC46050.032.91026742-43
GTAGGGG32200.031.4967118-19
GGGGCCC15050.030.30383944-45
AGGGGCG31900.030.2965410-11
ATCGGGA57000.029.9266822
CTCGGGG39450.029.67306336-37
GGGCGGG34350.029.45201312-13
TCGGGAG49900.029.4201893
GGGGGCG46900.028.10997840-41
GGGCCCG9050.027.2971744-45
AGGGTAG38800.026.81054328-29
TAGGGGA40500.025.51118-19
Produced by FastQC (version 0.11.2)